ELOVL5 gene variants reveal significant effects of milk quality in Holstein cows
The objective of this study was to evaluate the association between ELOVL5 gene polymorphisms and milk quality of Holstein cattle.Using direct DNA sequencing,seven single nucleotide polymorphisms(SNPs)and 1-bp del polymorphism were identified within the promoter region of ELOVL5 gene from 136 individuals.According to the statistical analysis,1-bp ins/del polymorphism was significantly associated with somatic cell count(SCC)(P＜0.01),and SNP7g.-385C＞G was significantly associated with urea nitrogen and corrected milk yield (P＜0.05).Then thetranscriptional activities of different genotypes were determined using a dual-luciferase reporter assay system in bovine mammary epithelial cells(MECs),the homozygous 1-bp del mutation genotype increased by a 1.59-fold(P＜0.01) activity of luciferase compared with the wild type.
NETL
NSTL
万方数据
维普
