Effects of oil origanum on performance and immunity of laying hens
This study was conducted to investigate the effects of different dose of oil origanum on the performance and immunity of laying hens.Three hundred Hy-Line ash layers aged 30 weeks were randomly assigned into 5 treatments(6 replicates per treatment,10 hens per replicate) for 60 days:negative control(basal diet only),groups A,B,and C(basal diet supplemented with 50,100 and 150 mg/kg oregano oil,respectively) and antibiotics group(basal diet plus 100 mg/kg of 10% colistin sulfate).The results showed as follows:in the whole experimental period,there was no significant difference in average daily feed intake among all groups(P>0.05),compared with control,group A and B significantly increased laying rate(P<0.05),reduced the ratio of feed to egg (P<0.05);serum immunoglobulin G content of group A,B and C was significantly higher than thag of control(P<0.05),at the 30th and 60th day of the experiment,newcastle viral antibody titer of group A was significantly increased(P<0.05);there was no significant difference in spleen index and percent of CD4+ among all groups(P>0.05),however,compared with control,group A significantly decreased percent of CD8+ and increased CD4+/CD8+.Moreover,the expression level of IL-2 and IL-4 mRNA in spleen were significant increased in group A(P<0.05).It is concluded that oil origanum can improve the performance and immune function of laying hens,and the 50 mg/kg oil origanum was a suitable dosage.