Isolation,identification and genome sequencing of BPIV3 genotype B
To investigate the infection of bovine parainfluenza virus type 3 in herd of calves aged 1-6 months in an intensive cattle farm in Xinjiang,60 nasal swab samples were subjected to RT-PCR assay,virus isolation and identification,whole genome sequencing and genetic evolutionary analy-sis.The results showed that the nucleic acid positivity rate of BPIV3 was 11.67％.A strain of BPIV3 was isolated from the nucleic acid positive sample and named XJ21032-1(45),its genome length was 15 512 bp.Genetic evolutionary analysis showed that XJ21032-1(45)belonged to BPIV3 genotype B,and the highest homology was 93.4％with the Australian BPIV3 genotype B reference strain Q5592(EU277658).This study successfully isolated a strain of B-genotype BPIV3,confir-ming the existence and prevalence of B-genotype strains in China,providing raw materials for the development of BPIV3 vaccines,and contributing to further research on the molecular evolution and traceability of BPIV3.
BPIV3genotype Bisolation and identificationgenome sequencing
NETL
NSTL
万方数据
