1株B基因型牛副流感3型病毒的分离鉴定及全基因组序列分析
Isolation,identification and genome sequencing of BPIV3 genotype B
张倩 1王旭 1韩猛立 1张星星 1吴桐忠 1钟发钢 1黄新 1张乾义2
作者信息
- 1. 新疆农垦科学院省部共建绵羊遗传改良与健康养殖国家重点实验室,新疆石河子 832000
- 2. 中国兽医药品监察所,北京 100081
- 折叠
摘要
为探究新疆某集约化牛场1～6月龄犊牛群中牛副流感病毒3型(BPIV3)的感染情况,对采集的60份犊牛鼻拭子样品进行RT-PCR检测、病毒分离鉴定及全基因组测序和遗传进化分析.结果显示,样品中BPIV3核酸阳性率为11.67％.从核酸阳性样品中分离获得1株BPIV3,并命名为XJ21032-1(45),其基因组全长为15 512 bp;遗传进化分析表明,XJ21032-1(45)属于BPIV3 B基因型,该分离株与澳大利亚的BPIV3 B基因型参考株Q5592(EU277658)的同源性最高为93.4％.本研究成功分离得到了 1株B基因型BPIV3,证实B型毒株在我国的存在和流行,为BPIV3疫苗研发提供了原材料,也有助于我国BPIV3分子进化规律及溯源的进一步研究.
Abstract
To investigate the infection of bovine parainfluenza virus type 3 in herd of calves aged 1-6 months in an intensive cattle farm in Xinjiang,60 nasal swab samples were subjected to RT-PCR assay,virus isolation and identification,whole genome sequencing and genetic evolutionary analy-sis.The results showed that the nucleic acid positivity rate of BPIV3 was 11.67％.A strain of BPIV3 was isolated from the nucleic acid positive sample and named XJ21032-1(45),its genome length was 15 512 bp.Genetic evolutionary analysis showed that XJ21032-1(45)belonged to BPIV3 genotype B,and the highest homology was 93.4％with the Australian BPIV3 genotype B reference strain Q5592(EU277658).This study successfully isolated a strain of B-genotype BPIV3,confir-ming the existence and prevalence of B-genotype strains in China,providing raw materials for the development of BPIV3 vaccines,and contributing to further research on the molecular evolution and traceability of BPIV3.
关键词
牛副流感病毒3型/B基因型/分离鉴定/全基因组序列分析Key words
BPIV3/genotype B/isolation and identification/genome sequencing引用本文复制引用
基金项目
新疆农垦科学院优秀中青年人才培养引导计划专项资助项目(SKLSGIHP2021A02)
新疆兵团重点领域科技攻关计划资助项目(2019AB029)
中央级公益性科研院所基本科研业务费院级统筹资助项目(Y2022LM21)
出版年
2024
NETL
NSTL
万方数据