摘要
为了解西藏林芝牦牛源产气荚膜梭菌的流行特点及耐药情况,为当地牦牛源产气荚膜梭菌病的防治提供理论基础和数据支持.对采集自西藏林芝市的87份牦牛腹泻粪便样品中的产气荚膜梭菌进行分离鉴定、毒素分型、药敏试验及耐药基因检测,并分析耐药基因与耐药表型的相关性.结果显示:从87份牦牛腹泻粪便样品中分离鉴定得到20株产气荚膜梭菌,分离率为22.99％;其中17株为A型,3株为D型.遗传进化分析发现Nyingchi-C12(OQ704284)与北京人源(KP944151.1)产气荚膜梭菌具有较近的亲缘性.分离株对氨基糖苷类药物耐药率分别为庆大霉素85.0％、卡那霉素90.0％,对多黏菌素B、磺胺甲噁唑的耐药率分别为80.0％、85.0％;对头孢唑啉、头孢噻吩、多西环素、米诺霉素100％敏感;35.0％的菌株4重耐药,甚至出现6重及7重耐药的情况.检测到qnrS(55％)、aac(6')-Jb(100％)、aac(6')/aph(2")(10％)、flor(95％)、blaTEM(10％)、tetM(45％)、tetA(P)(15％)、tetB(P)(10％)、sul2(65％)、sul3(20％)共10种耐药基因;抗磺胺类耐药基因与耐药表型的相符率最高,为70.6％.表明林芝市存在牦牛源产气荚膜梭菌的流行,并存在不同程度的耐药情况,提示我们需持续监测产气荚膜梭菌的流行及耐药情况,合理防控牦牛产气荚膜梭菌病.
Abstract
The aim of this study is to understand the epidemiological characteristics and drug resist-ance of Clostridium perfringens from yak in Nyingchi,Xizang,and provide theoretical basis and data support for the prevention and control of local yak-derived Clostridium perfringens.The iso-lation,toxin typing,drug sensitivity test and drug resistance gene detection of Clostridium per-fringens in 87 samples of yak diarrhea feces collected from Nyingchi City,Xizang were carried out,and the correlation between drug resistance gene and drug resistance phenotype was analyzed.The results showed that 20 strains of Clostridium perfringens were isolated and identified from 87 samples of yak diarrhea feces,and the isolation rate was 22.99％.Among them,17 were type A and 3 were type D.Genetic evolution analysis showed that Nyingchi-C12(OQ704284)was closely re-lated to Clostridium perfringens from Peking Man(KP944151.1).The resistance rates to amin-oglycosides were gentamicin 85.0％and kanamycin 90.0％,and the resistance rates to polymyxin B and sulfamethoxazole were 80.0％and 85.0％,respectively.It was 100％sensitive to cefzolin,cefthiophene,doxycycline and minomycin.35.0％of the strains were resistant to 4,and even to 6 and 7 antibiotics.The test detected qnrS(55％),aac(6')-Ib(100％),aac(6')/aph(2")(10％),flor(95％),blaTEM(10％),tetM(45％),tetA(P)(15％),tetB(P)(10％),sul2(65％)),sul3(20％),10 kinds of drug-resistant genes.The coincidence rate of anti-sulfanilamide resistance gene and drug-resistant phenotype was the highest(70.6％).The results indicated that the preva-lence and drug resistance of Clostridium perfringens from yaks existed in Nyingchi City,and that the prevalence and drug resistance of Clostridium perfringens should be continuously monitored to prevent and control clostridium perfringens in yaks reasonably.
基金项目
国家现代农业产业技术体系资助项目(CARS-37)
NETL
NSTL
万方数据