低氧环境紫锥菊提取物-菊苣酸通过PPAR信号通路对SD大鼠肝脏组织脂肪合成的影响
Effect of Echinacea purpurea extract-chicory acid on lipid synthesis in SD rat liv-er tissues via PPAR signaling pathway in hypoxic environment
行倩文 1吴华 1刘嘉华 1刘波 1张龙飞 1陈鑫磊 1马欣茹1
作者信息
- 1. 青海大学农牧学院,青海西宁 810016
- 折叠
摘要
为探究紫锥菊提取物-菊苣酸在低氧环境下影响SD大鼠肝脏脂肪代谢的分子机制.选取60只健康雄性SD大鼠(6～8周龄,(192±7.35)g)(P＞0.05),适应性喂养2周后,随机分为对照组、不同浓度CA组(10、20、40 mg/kg),每组15只,连续灌胃49 d.4组大鼠均自由采食和饮水,灌胃第50天对SD大鼠进行麻醉处理后,采集肝脏组织,并通过油红O染色法计算各组大鼠肝脏组织油滴面积占总面积的百分比以及测定PPAR信号通路关键因子PLIN2、PLIN5、PPARα、PPARγ、RXRα的表达情况.油红O染色结果显示:与对照组相比,添加紫锥菊提取物-菊苣酸组极显著降低油红O染色面积(P＜0.01);PPAR信号通路关键因子表达结果显示:与对照组相比,添加紫锥菊提取物-菊苣酸组显著降低PLIN2、PLIN5、PPARα、PPARγ、RXRα的mRNA及蛋白表达量(P＜0.05),其中以添加10 mg/kg紫锥菊提取物-菊苣酸组对PLIN2、PLIN5、PPARα、PPARγ、RXRα的mRNA及蛋白表达量效果最显著(P＜0.05).结果表明,紫锥菊提取物-菊苣酸降低脂肪合成相关因子的表达,调控脂肪的合成与储存,预防脂肪的过多累积导致机体脂质代谢紊乱,紫锥菊提取物-菊苣酸改善了低氧诱导的脂质代谢功能障碍,且10 mg/kg组作用效果最好.本研究为紫锥菊提取物-菊苣酸作为高海拔地区预防高原动物脂质代谢紊乱的饲料添加剂的开发利用提供了理论基础.
Abstract
The aim of this study is to explore the molecular mechanism of Echinacea purpurea ex-tract-chicric acid effect on hepatic fat metabolism in SD rats.Sixty 6-8 weeks old healthy male SD rats(192±7.35)g were randomly divided into the control and CA groups(10,20,40 mg/kg)after 2 weeks of adaptive feeding.The liver tissues of the rats in each group were collected and the per-centage of the total area of oil droplets and the expression of the key factors of the PPAR signaling pathway including PLIN2,PLIN5,PPARα,PPARy and RXRα,were measured by Oil-Red O stai-ning.The results of Oil-Red O staining showed that the area of staining was significantly reduced in the group with the addition of Echinacea extract-chicory acid compared with the control group(P＜0.01);the results of expression of key factors of PPAR signaling pathway showed that the expression of PLIN2,PLIN5,PPARα,PPARy and RXRα was significantly reduced in the group with the addition of Echinacea extract-chicory acid compared with the control group(P＜0.05).The effect of adding 20 mg/kg of Echinacea extract-chicory acid on the mRNA and protein expres-sion of PLIN2,PLIN5,PPARα,PPARy and RXRα was the most significant(P＜0.05).In conclu-sion,Echinacea extract-chicory acid reduced the expression of lipid synthesis-related factors,regu-lated lipid synthesis and storage,and prevented the excessive accumulation of fat leading to the disorder of lipid metabolism in the body,and Echinacea extract-chicory acid improved the dysfunction of lipid metabolism induced by hypoxia,and the 20 mg/kg group had the best effect.This study may provide a theoretical basis for the development and utilization of Echinacea ex-tract-chicric acid as a feed additive to prevent lipid metabolism disorders in highland animals at high altitude.
关键词
低氧/菊苣酸/SD大鼠/肝脏组织/油红O/PPAR信号通路/脂肪合成Key words
hypoxia/chicory acid/SD rats/liver tissues/Oil-Red O/PPAR signaling pathway/lipid synthesis引用本文复制引用
基金项目
国家自然科学基金资助项目(32160812)
出版年
2024
NETL
NSTL
万方数据