摘要
为了探讨翁芩复方(WQ)对雏鸭感染禽多杀性巴氏杆菌(Pm)的预防效果及其作用,本研究采用肉汤二倍稀释法体外测定最小抑菌浓度(MIC)和最小杀菌浓度(MBC);灌胃氟苯尼考(FF)及不同剂量WQ预处理雏鸭7 d后口服感染Pm,通过比较日增质量和成活率评价 WQ对Pm感染雏鸭的预防效果,RT-PCR和细菌计数检测Pm入侵和定植肝脏程度,全血细胞计数(CBC)检测血液中血细胞变化,RT-qPCR检测十二指肠白介素6(IL-6)、肿瘤坏死因子α(TNF-α)、白介素10(IL-10)、白介素4(IL-4)表达.结果 表明:WQ对Pm有明显的体外抑菌效果;WQ干预后雏鸭成活率提高,日增质量提高;WQ组肝脏的Pm数量减少;WQ能显著改善血红蛋白浓度(HGB)和红细胞压积(HCT);WQ降低Pm诱导的IL-10和IL-4水平.因此,WQ可有效预防Pm感染,为临床防控该菌大规模流行提供了技术支撑.
Abstract
To investigate the preventive effect of Wengqin decoction(WQ)in Pasteurella multoci-da(Pm)infected ducklings,the minimal inhibitory concentration(MIC)and minimum bactericid-al concentration(MBC)were determined by tube dilution;ducklings were pretreated with florfeni-col(FF)and WQ by gavage for 7 d and then orally infected with Pm,the protective effect of WQ was assessed by comparing daily gain and mortality rate,colonization and internalization of Pm in-to liver was detected by RT-PCR and bacterial count,hemocyte changes was detected by complete blood count(CBC),and the expression of IL-6,TNF-α,IL-10 and IL-4 in duodenum were detected by RT-qPCR.The results showed that Pm was sensitive to WQ;the mortality rate decreased,and Green-Eco-Healthy Animal Husbandry of Zhejiang Province/Zhejiang Provincial Research Cen-ter for Animal Health Diagnostics & Advanced Technology/Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management/China-Australia Joint Laboratory for Animal Health Big Data Analytics,College of Animal Science and Technol-ogy & College of Veterinary Medicine of Zhejiang A&F University,Hangzhou 311300,China;2.Faculty of Agriculture,Jinhua Polytechnic,Jinhua,Zhejiang 321007,China;3.Shaoxing Xian-heng Duck Breeding Co.,Ltd.,Shaoxing,Zhejiang 312000,China)the daily gain increased in Pm-infected ducklings after WQ intervention,and the number of Pm present in liver decreased,hemoglobin(HGB)and hematocrit(HCT)levels were recovered,and the gene expression of Pm-induced IL-10 and IL-4 was fully suppressed.In conclusion,WQ can ef-fectively protect ducklings from Pm infection,and provided technical support for epidemic preven-tion and control of Pm.
基金项目
浙江省三农六方资助项目(2021SNLF023)
金华市科技计划资助项目(2022-2-022)
国家自然科学基金青年科学基金资助项目(32102711)
浙江农林大学科研发展基金资助项目(2021FR003)
NETL
NSTL
万方数据