Effects of IGF-1 c.258 synonymous mutations on osteoclast proliferation,differen-tiation and related pathways
Studies have found that the synonymous mutation of IGF-1 affects bone formation and resorption,and osteoclasts are the key to mediate bone resorption.In order to analyze the effect of synonymous mutation on osteoclast proliferation,differentiation and related pathways,the mice with synonymous mutation of IGF-1 were used as the experimental subjects.Osteoclast precursor cells were isolated and cultured,and osteoclast differentiation was induced to detect the prolifera-tion and bone resorption function of different genotypes of osteoclasts.The results showed that the expression of IGF-1 in OCL-G was significantly higher than that in OCL-A(P＜0.01).There was no significant difference in gene expression between NFATc1 and LPAR(P＞0.05).However,the ephrinB2 gene expression was higher in OCL-G(P＜0.01).The expression of Sema4D gene was also higher in OCL-G(P＜0.001).At6v0d2 showed the opposite pattern(P＜0.001).There was no significant change in the proliferation of osteoclasts and bone resorption function.In summary,IGF-1 synonymous mutation affects the expression of IGF gene and related pathway genes.It also explains the reason why there is no significant change in osteoclast proliferation and bone resorp-tion,which lay a foundation for further study on the effect of IGF-1 synonymous mutation on oste-oclasts.
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