Establishment and preliminary application of TaqMan fluorescence quantitative detection method for porcine rotavirus
In order to monitor the early porcine rotavirus infection,according to the existing porcine rotavirus A VP6 sequence in GenBank,two pairs of primers and fluorescence probes were de-signed,and the TaqMan real-time RT-PCR method was established,and the specificity,sensitivity and repeatability of the method were evaluated.The results showed that the established TaqMan real-time RT-PCR method had strong specificity,good repeatability and high sensitivity,and could detect 101 copies/μL.After testing the fecal samples of 2 366 diarrheal piglets,the detection rate of the established method(5.92％,140/2 366)was higher than that of conventional RT-PCR(4.95％,117/2 366),and the compliance rate of the two was 98.58％.The results showed that the TaqMan fluorescence RT-PCR method established in this study can be used for the clinical detec-tion of porcine rotavirus disease.
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