A novel electrochemical immunosensor for detection of fowl adenovirus serotype 4
The aim of this study is to construct a novel resistive electrochemical immunosensor for the detection of fowl adenovirus serotype 4(FAdV-4).Multi-walled carbon nanotubes-chitosan-Pt nanoparticles(MWCNT-Chi-PtNP)was used to modify gold electrode(GE),then adsorbed with FAdV-4 penton protein monoclonal antibody(MAb/FAdV-4)to construct electrochemical immu-nosensor for detection of FAdV-4.After optimizing the test substrate and incubation time of the sample,the sensitivity,specificity,and stability of the constructed electrochemical immunosensor were determined under the optimal conditions.Thirty-six clinical samples were detected by the con-structed electrochemical immunosensor to verify the practicability of the sensor.Results showed that the optimal pH value of the electrolyte was 7.0,the optimal incubation time of the samples was 30 min.The developed electrochemical immunosensor showed a wide linear range from 101,46 EID50/mL to 104.46 EID50/mL and a detection limit of 10189 EID50/mL FAdV-4(S/N=3),the sen-sitivity of the developed electrochemical immunosensor was 37 times higher than that of the con-ventional PCR(103,46 EID50/mL).This sandwich electrochemical immunosensor also exhibited high selectivity and stability.The results of 36 clinical sample detected by the developed electro-chemical immunosensor were consistent with the results of PCR and sequencing.The established electrochemical immunosensor has the advantages of high sensitivity,good specificity,and rapid detection,which provides a new rapid detection for the diagnosis FAdV-4.
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