鸭短喙矮小综合征灭活疫苗的研制及免疫效力评价
Development and evaluation of the immune efficacy of an inactivated vaccine a-gainst duckling short beak dwarfism syndrome
陈仕龙 1程晓霞 1肖世峰 1江丹丹 1林锋强 1王劭 1朱小丽 1俞伏松 2邹敏 3陈少莺1
作者信息
- 1. 福建省农业科学院畜牧兽医研究所,福建福州 350000;福建省畜禽疫病防治工程技术研究中心,福建福州 350013
- 2. 福建省畜禽疫病防治工程技术研究中心,福建福州 350013
- 3. 青岛易邦生物工程有限公司,山东青岛 266000
- 折叠
摘要
鸭短喙矮小综合征(short beak and dwarfism syndrome,SBDS)是近年来在我国鸭群中流行的一种新传染病,为研制SBDS灭活疫苗,本研究将SBDSV M15株在番鸭胚增殖,收获病毒尿囊液经灭活后制备了 3批灭活疫苗,并对疫苗的安全性、免疫原性及免疫效力进行检验.结果显示,3 日龄樱桃谷鸭超剂量接种1.0 mL/羽,未见不良反应,表明疫苗的安全性好;3批灭活疫苗免疫20日龄雏半番鸭,免疫后28 d血清的胶乳凝集抑制试验(LPAI)抗体效价均值为4.3 log2;130日龄樱桃谷鸭种鸭免疫1次灭活疫苗后60 d血清LPAI抗体平均效价为6.9 log2;种鸭免疫后60 d所产种蛋卵黄抗体的LPAI效价均值为6.5 log2;对种鸭免疫后的3日龄子代雏鸭进行攻毒获得100％免疫保护.结果表明,本研究研制的SBDS灭活疫苗具有良好的免疫原性,种鸭1次免疫后可提供子代雏鸭保护性母源抗体,保护雏鸭度过最易感的危险窗口期,为SBDS的防控提供了有效手段.
Abstract
Short beak and dwarfism syndrome(SBDS)is an infectious disease prevalent in Chinese duck flocks in recent years.In order to develop a SBDS inactivated vaccine,SBDSV M15 strain was inoculated in Muscovy duck embryos,and the allantoic fluid was harvested and inactivated by formaldehyde.Three batches of oil adjuvant inactivated vaccine were prepared,and the safety,im-munogenicity and immune efficacy of this vaccine was evaluated.The results showed that the vac-cine has good safety to 3-day-old Cherry Valley ducklings,no any adverse reactions were observed after injection an overdose of 1 mL per duck.Twenty-day-old mule duckling were immunized with three batches of inactivated vaccine and the mean sera antibody titer detected by latex particle ag-glutination inhibition assay(LPAI)was 4.3 log2 at 28 days post vaccination.High level of LPAI antibody was also induced in 130-day-old breed Cherry valley ducks with one dose vaccination,the average titers of LPAI antibody in sera and egg yolk were 6.9 log2 and 6.5 log2 at 60 d respectively.Complete immune protection was obtained when 3-day-old offspring ducks containing maternal antibodies were challenged with the pathogenic strain SBDSV M15.Taken together,the inactivated SBDS vaccine developed in this study has good immunogenicity,breed ducks with a single immuni-zation could offer offspring ducklings with high level of maternal antibodies to protect the duck-lings against SBDSV infection through the susceptibility period,providing an effective means for the prevention and control of SBDS.
关键词
鸭短喙矮小综合征/灭活疫苗/抗体效价/母源抗体/免疫效力Key words
duckling short beak and dwarfism syndrome/inactivated vaccine/antibody titer/mater-nal antibody/immune efficacy引用本文复制引用
基金项目
中央引导地方科技发展专项(2022L3019)
福建省农科院动物新发病毒病防控科技创新团队项目(CXTD2021019-2)
福建省农科院"5511"协同创新工程项目(XTCXGC2021012)
福建省农科院"5511"协同创新工程项目(XTCXGC2021018)
福建省公益类科研院所专项(2021R10260010)
福建省科技重大专项(2019NZ09008)
出版年
2024
NETL
NSTL
万方数据