To study the biological characteristics and gene function of Ligilactobacillus salivarius CICC23174 strain,a strain was isolated from chicken intestines.The next generation sequencing platform HiSeq2000 was used for sequencing,and bioinformatics software was employed to assem-ble and optimize the raw data.Functional annotation,bacteriocin and signal peptide,gene collineari-ty comparison,and phylogenetic analysis were performed on its gene information.The results indi-cated that the linear genome of Ligilactobacillus salivarius CICC23174 strain was 203 542 bp in length,with a GC content of 32.84％.encoding 1 890 genes,containing 3 bacteriocins,and 50 signal peptides.Importantly,the Ligilactobacillus salivarius CICC23174 strain included 27 unique genes,forming four systems:the Type Ⅰ CRISPR-Cas defense system,the Type Ⅱ toxin antitoxin sys-tem,the tumor inhibitory gene,and the protein secretion pathway.Moreover,collinearity analysis showed that CICC23174 strain was most similar to the Ligilactobacillus salivarius UCC118 strain,but 16S rRNA phylogenetic analysis indicted that CICC23174 strain is genetically close to FZJTZ9M6 strain.This study completed the species evolution library of Ligilactobacillus salivari-us,and also lay the foundation for the investigation into the potential probiotic functions of Ligi-lactobacillus salivarius.
万方数据