Effects and mechanisms of 10-hydroxydecanoic acid on polycystic ovary syndrome in mice
To investigate the effects of 10-hydroxydecanoic acid(10-HDA)on polycystic ovary syn-drome(PCOS)mice,21-day-old ICR mice were selected to establish the PCOS model through subcutaneous injection of dehydroepiandrosterone(DHEA),followed by the treatment with 10-HDA.The effects of 10-HDA on estrous cycle,ovarian morphology,hormone secretion,and oxida-tive stress of PCOS mice were evaluated using vaginal smears,HE staining,ELISA,real-time fluo-rescence quantitative PCR,etc.The results showed that after administration of 10-HDA into PCOS mice,estrous cycle was restored,and the number of cystic follicles significantly decreased.Mean-while,10-HAD diminished the serum testosterone(T)level and restricted the expression of an-drogen synthesis gene Cyp17a1(cytochrome P450,family 17,subfamily a,polypeptide 1)in PCOS mice ovaries.Additionally,10-HDA reduced the level of luteinizing hormone(LH)in the serum form PCOS mice but increased the level of serum follicle-stimulating hormone(FSH)together with the decline for LH/FSH ratio.Moreover,10-HDA improved the levels of estrogen and proges-terone in the serum from PCOS mice,promoted the expression of antioxidant stress-related genes Nrf2 and Foxo1,and weakened the oxidative stress in PCOS mice.In conclusion,these results dem-onstrated that 10-HDA has significant therapeutic effects on PCOS mice.
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