布劳恩脂蛋白耐受对大肠杆菌感染诱导奶牛子宫内膜炎症反应的调控作用
Modulatory role of Braun lipoprotein tolerance on endometrial inflammatory re-sponse to Escherichia coli infection in dairy cows
于琢雅 1巩志国 1任佩佩 1赵佳敏 1毛伟 1张双翼 1刘谕泽 1吴敬泽 1刘博1
作者信息
- 1. 内蒙古农业大学兽医学院,内蒙古呼和浩特 010018
- 折叠
摘要
子宫内膜炎是奶牛常见的生殖障碍疾病,大肠杆菌是引起该病的主要病原微生物.耐受是宿主的一种防御策略,可以减少感染对宿主适应性的负面影响.但布劳恩脂蛋白(Braun lipoprotein,BLP)耐受是否对大肠杆菌感染诱导的奶牛子宫内膜炎具有调控作用 目前尚不清楚.本研究分析了 BLP对大肠杆菌感染后子宫内膜上皮细胞和子宫内膜组织中炎症介质分泌的影响,以及BLP对子宫内膜组织中HMGB1的表达水平.此外,探究了 BLP对子宫内膜上皮细胞中信号通路激活的影响.试验结果表明,BLP的存在可显著下调脂多糖(lipopolysaccharide,LPS)或大肠杆菌诱导的奶牛子宫内膜上皮细胞和子宫内膜组织中促炎性细胞因子的分泌水平,同时上调抗炎因子分泌水平.免疫荧光结果表明,BLP可显著降低奶牛子宫内膜组织中HMGB1的表达.值得注意的是,BLP的存在可显著下调LPS或大肠杆菌刺激诱导的MAPK p38和NF-κB p65的磷酸化水平.结果表明,BLP耐受在大肠杆菌诱导的子宫内膜炎中起着至关重要的作用,BLP耐受可显著降低奶牛子宫组织的炎症反应.
Abstract
Endometritis is a common reproductive disorder in dairy cows,and Escherichia coli(E.coli)is the main pathogenic microorganism causing the disease.Tolerance is a defensive strategy of the host to reduce the harmful effects of infection on the host's fitness.However,whether Braun lipoprotein(BLP)tolerance has a regulatory effect on E.coli infection-induced cow endometritis remains unclear.This study analyzed the effects of BLP on the secretion of inflammatory mediators in endometrial epithelial cells and endometrial tissues after E.coli infection,as well as the expres-sion level of HMGB1 in endometrial tissues.In addition,the effects of BLP on the activation of sig-naling pathways in endometrial epithelial cells were investigated.The results showed that BLP could significantly down-regulate the secretion of pro-inflammatory cytokines and up-regulate the secretion of anti-inflammatory cytokines in the endometrial epithelial cells and endometrial tissues of dairy cows induced by LPS or E.coli.Immunofluorescence results showed that BLP could signif-icantly reduce the expression of HMGB1 in cow endometrial tissue.Notably,BLP can significantly down-regulate the phosphorylation levels of MAPK p38 and NF-κB p65 induced by LPS or E.coli.The results indicated that BLP tolerance plays a crucial role in E.coli-induced endometritis,and BLP tolerance can significantly reduce the inflammatory response in cow uterine tissue.
关键词
耐受/奶牛子宫内膜炎/大肠杆菌/布劳恩脂蛋白/脂多糖Key words
tolerance/bovine endometritis/Escherichia coli/Braun lipoprotein/lipopolysaccharide引用本文复制引用
出版年
2024
NETL
NSTL
万方数据