Modulatory role of Braun lipoprotein tolerance on endometrial inflammatory re-sponse to Escherichia coli infection in dairy cows
Endometritis is a common reproductive disorder in dairy cows,and Escherichia coli(E.coli)is the main pathogenic microorganism causing the disease.Tolerance is a defensive strategy of the host to reduce the harmful effects of infection on the host's fitness.However,whether Braun lipoprotein(BLP)tolerance has a regulatory effect on E.coli infection-induced cow endometritis remains unclear.This study analyzed the effects of BLP on the secretion of inflammatory mediators in endometrial epithelial cells and endometrial tissues after E.coli infection,as well as the expres-sion level of HMGB1 in endometrial tissues.In addition,the effects of BLP on the activation of sig-naling pathways in endometrial epithelial cells were investigated.The results showed that BLP could significantly down-regulate the secretion of pro-inflammatory cytokines and up-regulate the secretion of anti-inflammatory cytokines in the endometrial epithelial cells and endometrial tissues of dairy cows induced by LPS or E.coli.Immunofluorescence results showed that BLP could signif-icantly reduce the expression of HMGB1 in cow endometrial tissue.Notably,BLP can significantly down-regulate the phosphorylation levels of MAPK p38 and NF-κB p65 induced by LPS or E.coli.The results indicated that BLP tolerance plays a crucial role in E.coli-induced endometritis,and BLP tolerance can significantly reduce the inflammatory response in cow uterine tissue.
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