羊口疮重组活病毒载体研究进展
Advances in recombinant live viral vector of orf
李河林 1张雅雅 2王田田 2王运航 2魏佳彤 2兰梦繁 2薛青青 2张睿 2敬晓棋2
作者信息
- 1. 榆林学院生命科学学院,陕西榆林 719000;榆林学院陕北绒山羊工程技术研究中心陕北羊产业发展协同创新中心,陕西榆林 719000
- 2. 榆林学院生命科学学院,陕西榆林 719000
- 折叠
摘要
重组病毒载体是当前病毒基因工程研究的热点之一.羊口疮(orf),也称羊传染性脓疱皮炎,是由羊口疮病毒(orf virus,ORFV)引起的急性、接触性、嗜上皮性人畜共患传染病.ORFV为痘病毒科(Poxviridae)、脊索动物痘病毒亚科(Chordopoxrinae)的副痘病毒属(Parapoxvirus)成员,线性双股DNA病毒,病毒基因组较大,改造后可容纳较大的外源基因,适合开发为活病毒载体.本文综述了orf重组活病毒载体的优势、构建策略及其应用研究的最新进展,为ORFV研究、重组活病毒开发应用提供参考.
Abstract
Recombinant virus vector has become one of the current hotspots in viral genetic engi-neering.Orf,also known as contagious pustular dermatitis,is an acute,contact,epitheliophilic zoo-noses that is caused by orf virus(ORFV),the type species of the genus Parapoxvirus within the poxvirus subfamily Chordopoxvirinae.ORFV is linear double-stranded DNA(dsDNA)virus with a large viral genome that can accommodate large exogenous genes after transformation.It is suit-able for developing as a live viral vector.In this paper,the advantages,vector modification strate-gies,and application of recombinant live viral vector were reviewed,which can provide reference for the research of ORFV and the development and application of recombinant live virus.
关键词
羊口疮病毒/载体/重组病毒/基因工程Key words
orf virus/vector/recombinant virus/genetic engineering引用本文复制引用
基金项目
陕西省科技厅重点研发基金(2023-YBNY-143)
榆林学院高层次人才科研启动基金(20GK15)
出版年
2024
NETL
NSTL
万方数据