鼠伤寒沙门菌oppCDF基因缺失株的构建及生物学特性分析
Construction and biological characterization of oppCDF gene deficient strain of Salmonella typhimurium
杨婉 1张家莉 1陈世雄 1武绍碧 1叶景芬 1潘永 2杨琦1
作者信息
- 1. 贵州大学动物科学学院,贵州贵阳 550025
- 2. 贵州省农业科学院畜牧兽医研究所,贵州贵阳 550005
- 折叠
摘要
利用λ-Red同源重组技术构建鼠伤寒沙门菌oppCDF基因缺失株,并检测其生长特性、运动性、生物被膜形成能力、胞内存活能力及LD50毒力.结果显示,与野生型菌株相比,oppCDF基因缺失株的生长特性无明显变化;op-pCDF基因缺失株可降低鼠伤寒沙门菌的运动性;oppC与oppF基因缺失后可提高鼠伤寒沙门菌生物被膜形成能力;同时,oppC基因缺失可降低在RAW267.4细胞内的存活能力和其在小鼠体内的毒力,oppF基因缺失可显著增强在RAW267.4内的存活能力和对小鼠毒力的影响,而oppD基因缺失后其在小鼠体内的毒力和RAW267.4内的存活率均无显著变化.研究表明,oppCDF基因与鼠伤寒沙门菌的运动性、生物被膜形成和毒力密切相关,为进一步揭示鼠伤寒沙门菌致病作用中的复杂调控和机制提供了理论基础.
Abstract
The aim is to study the effect of oppCDF gene on the biological characteristics and path-ogenicity of Salmonella typhimurium(S.typhimurium).In this study,the oppCDF gene deletion strain of S.typhimurium was constructed by λ-Red homologous recombination technique,and its growth characteristics,motility,biofilm formation ability,intracellular survival ability and LD50 virulence were detected.The results showed that the growth characteristics of oppCDF gene dele-tion strains had no significant changes compared with the wild-type strains.oppCDF gene deletion can reduce the motility of S.typhimurium and affect the drug resistance of S.typhimurium in dif-ferent degrees.Deletion of oppC and oppF genes improved the biofilm formation ability of S.ty-phimurium.At the same time,oppC gene deletion reduced the survival ability in RAW267.4 cells and its virulence in mice,oppF gene deletion significantly enhanced the survival ability in RAW267.4 cells and its influence on the virulence of mice,while oppD gene deletion had no signif-icant changes in the virulence and survival rate in mice RAW267.4 cells.Studies have shown that oppCDF gene is closely related to the motility,biofilm formation and virulence of S.typhimurium.These results provide a theoretical basis for further revealing the complex regulation and mecha-nism of S.typhimurium pathogenicity.
关键词
鼠伤寒沙门菌/λ-Red同源重组/oppCDF基因/生物学特性Key words
Salmonella typhimurium/λ-Red homologous recombination/oppCDF gene/biological characteristics引用本文复制引用
出版年
2024
NETL
NSTL
万方数据