Peptidoglycan-associated lipoprotein affects biofilm formation and drug resistance of Salmonella enterica
In order to investigate the role of peptidoglycan-associated lipoprotein(Pal)in biofilm formation and drug resistance of Salmonella enterica,a pal-deficient strain of Salmonella enterica was constructed,and its ability to form biofilm,the formation of Curli hairs and fibrils and the ex-pression levels of biofilm-related genes were examined.The results showed that compared with the wild-type strain the pal-deficient strain had significantly lower biofilm formation ability,lower se-cretion of Curli hairs,but no significant reduction in cellulose formation,and significantly lower ex-pression levels of biofilm-related genes.To further analyze the role of pal gene in Salmonella en-terica drug resistance,the lowest inhibitory concentration of polymyxin B against this bacterium was tested.The results showed that the pal-deficient strain showed approximately 75％lower MIC against polymyxin B.In summary,this study showed that the pal gene affects Salmonella enterica biofilm formation and drug resistance,and the results lay the foundation for functional studies of Salmonella enterica Pal.
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