Effect of Echinococcus granulosus infection on liver fibrosis in mice
The aim of this study is to investigate the effects of different doses of Echinococcus gran-ulosus in infection at different times on host liver fibrosis.The model of high and low doses(2 000 and 500 protoscoleces)of Echinococcus granulosus in BALB/c mice was established by portal vein injection.The liver specimens of mice were collected after 1,3 and 8 months,respectively.The de-gree of fibrosis was identified by Masson staining,and the expression of α-smooth muscle actin(α-SMA)and type Ⅲ collagen(collagen Ⅲ)was detected by immunohistochemistry.Masson staining showed that the collagen staining of the liver tissue at the edge of the lesion was significantly high-er than that of the normal liver tissue and formed a dense ring structure,which was widely distributed around the capsule wall.Compared with the control group,the degree of fibrosis in the experimental group was statistically different(P＜0.01).Immunohistochemical(α-SMA)staining showed that the liver of the experimental group was mainly expressed in the area of inflammatory infiltration and granulation tissue hyperplasia.Compared with the control group,the expression ofα-SMA in the experimental group was statistically different(P＜0.01).The results of immunohis-tochemistry(collagen Ⅲ)staining showed that collagen Ⅲ was mainly expressed around the blood vessels in normal liver tissue,while it was mainly expressed around the lesions in the liver of mice in the low-and high-dose Echinococcus granulosus infection group.Compared with the control group,there were statistical differences between the high-dose group and the low-dose group at different time points(P＜0.001).In conclusion,the host infected with Echinococcus granulosus can lead to liver fibrosis,and the expression levels of α-SMA and collagen Ⅲ in the liver of mice in-fected with different doses of Echinococcus granulosus at different time points increase with the in-crease of collagen area.
Echinococcus granulosusα-smooth muscle actintype m collagenliver fibrosis
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