细粒棘球蚴感染对小鼠肝纤维化的影响
Effect of Echinococcus granulosus infection on liver fibrosis in mice
玛伊热·艾则孜 1何荣东 2段永只 3朱江 2周檀芳 2瓦热斯·吐尔松 1地里木拉提·热衣木江 4赛福丁·阿不拉 1卡力比夏提·艾木拉江 5阿得力江·吾斯曼1
作者信息
- 1. 新疆农业大学 动物医学学院,新疆乌鲁木齐 830052
- 2. 新疆医科大学第一附属医院临床医学研究院省部共建中亚高发病成因与防治国家重点实验室,新疆乌鲁木齐 830054
- 3. 博乐市畜牧兽医发展中心,新疆博乐 833499
- 4. 新疆畜牧科学院畜牧业经济与信息研究所,新疆乌鲁木齐 830099
- 5. 新疆农业大学 动物医学学院,新疆乌鲁木齐 830052;新疆医科大学第一附属医院临床医学研究院省部共建中亚高发病成因与防治国家重点实验室,新疆乌鲁木齐 830054
- 折叠
摘要
采用门静脉注射法建立细粒棘球蚴BALB/c小鼠高、低剂量(2 000、500原头蚴)肝脏模型,1、3及8个月后采集小鼠肝脏标本,Masson染色鉴定纤维化程度,免疫组化法检测α-平滑肌肌动蛋白(α-SMA)和Ⅲ型胶原蛋白(Colla-gen Ⅲ)的表达.Masson染色结果显示,病灶边缘带肝脏组织的胶原染色比正常肝脏组织明显增多并形成了致密的环状结构,在囊壁周围广泛分布,且与对照组相比,试验组纤维化程度具有统计学差异(P＜0.01);免疫组化(α-SMA)染色显示,试验组小鼠肝脏中α-SMA主要表达在炎症浸润及肉芽组织增生区域,与对照组相比,试验组α-SMA的表达量具有统计学差异(P＜0.01);免疫组化(Collagen Ⅲ)染色结果显示,正常肝脏组织中Collagen Ⅲ主要表达在血管周围,而在低剂量和高剂量细粒棘球蚴感染组小鼠肝脏中主要表达在病灶周围,且与对照组相比,不同时间点高剂量组和低剂量组具有统计学差异(P＜0.001).综上细粒棘球蚴感染小鼠可导致肝纤维化,且不同时间点不同剂量细粒棘球蚴感染小鼠肝脏中α-SMA、Collagen Ⅲ的表达水平随着胶原面积的增加而升高.
Abstract
The aim of this study is to investigate the effects of different doses of Echinococcus gran-ulosus in infection at different times on host liver fibrosis.The model of high and low doses(2 000 and 500 protoscoleces)of Echinococcus granulosus in BALB/c mice was established by portal vein injection.The liver specimens of mice were collected after 1,3 and 8 months,respectively.The de-gree of fibrosis was identified by Masson staining,and the expression of α-smooth muscle actin(α-SMA)and type Ⅲ collagen(collagen Ⅲ)was detected by immunohistochemistry.Masson staining showed that the collagen staining of the liver tissue at the edge of the lesion was significantly high-er than that of the normal liver tissue and formed a dense ring structure,which was widely distributed around the capsule wall.Compared with the control group,the degree of fibrosis in the experimental group was statistically different(P＜0.01).Immunohistochemical(α-SMA)staining showed that the liver of the experimental group was mainly expressed in the area of inflammatory infiltration and granulation tissue hyperplasia.Compared with the control group,the expression ofα-SMA in the experimental group was statistically different(P＜0.01).The results of immunohis-tochemistry(collagen Ⅲ)staining showed that collagen Ⅲ was mainly expressed around the blood vessels in normal liver tissue,while it was mainly expressed around the lesions in the liver of mice in the low-and high-dose Echinococcus granulosus infection group.Compared with the control group,there were statistical differences between the high-dose group and the low-dose group at different time points(P＜0.001).In conclusion,the host infected with Echinococcus granulosus can lead to liver fibrosis,and the expression levels of α-SMA and collagen Ⅲ in the liver of mice in-fected with different doses of Echinococcus granulosus at different time points increase with the in-crease of collagen area.
关键词
细粒棘球蚴/α-平滑肌肌动蛋白/Ⅲ型胶原蛋白/肝纤维化Key words
Echinococcus granulosus/α-smooth muscle actin/type m collagen/liver fibrosis引用本文复制引用
基金项目
国家自然科学基金(82160395)
中国博士后科学基金(2022MD723847)
新疆维吾尔自治区重点研发计划子课题(2022B03013-4)
省部共建中亚高发病成因与防治国家重点实验室开放基金(SKL-HIDCA-2021-JH7)
出版年
2024
NETL
NSTL
万方数据