Protective effect of ginsenosides Rg1 and Re on LPS-induced intestinal barrier damage
To investigate the protective effects of ginsenosides Rg1 and Re on LPS-induced intesti-nal barrier damage,40 4-week-old male Kunming mice were randomly divided into Control,LPS,Rg1 and Re groups,with 10 mice in each group.The Control and LPS groups were injected intrap-eritoneally with sterile PBS,the Rg1 group was injected intraperitoneally with Rg1,and the Re group was injected intraperitoneally with Re.On the 8th day of the experiment,the Control group was injected intraperitoneally with sterile PBS,and the rest of the groups were injected intraperito-neally with LPS,mice were executed after 6 h,sampled and examined.The results showed that:af-ter LPS treatment,DAI score increased significantly,liver,spleen and lung indices increased signif-icantly;jejunal and ileal villus length significantly shortened,jejunal and ileal crypt depth in-creased,and the ratio of villus length to crypt depth decreased;the protein expression of the jejunal and ileal tight junction protein Occludin significantly decreased;the mRNAs of TLR4,NF-κB and Caspase-1 mRNA expression levels significantly up-regulated.Whereas Rg1 and Re pretreatment attenuated the LPS-induced increase in DAI scores and decreased liver and lung indices;significant-ly increased villus length;Re significantly decreased jejunal crypt depth,and Rg1 and Re signifi-cantly increased the ratio of villus length to crypt depth;Rg1 pretreatment significantly increased the protein expression of ileal Occludin,and Re pretreatment significantly increased jejunal and ile-al Occludin;Rg1 pretreatment significantly down-regulated the mRNA expression levels of TLR4,NF-κB and Caspase-1;Re pretreatment significantly down-regulated the mRNA expression levels of TLR4 and Caspase-1.In summary,ginsenoside Rg1 and Re pretreatment improved intestinal morphology,increased tight junction protein expression,and inhibited mRNA expression of genes related to inflammation and apoptosis.
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