Oat derived β-glucan enhances immune protection of crude antigen against C57BL/6J mice infected by Trichinella spiralis via Dectin-1
Studies have shown that Dectin-1 is one of the important ways for BG to activate innate immunity and is involved in the infection process generated by various pathogens.It is a typical pattern recognition receptor,but whether BG enhances the immune response induced by crude an-tigens of Trichinella through dectin-1 pathway remains unclear.Forty-eight female C57BL/6J mice were randomly divided into four groups:the PBS control group;crude antigen(CA)treatment group;BG and CA treatment group;BG,CA and Dectin-1 neutralizing antibody(2A11)treatment group,with 12 animals in each group.The PBS control group was only immunized subcutaneously,and the CA group,BG+CA group and BG+CA+2 A11 groups were immunized subcutaneously three times every two weeks.Two weeks after the last immunization,the same amount of T.spira-lis was given orally in all groups.On the 7th day after infection,the parasite load of PBS control group,CA treatment group,BG+CA treatment group and BG+CA+2A11 treatment group and the parasite load of muscle larvae at the 35th day were counted.The expression of Dectin-1 gene was detected by qPCR on the 7th day after infection.IgG titers were measured 2,4,6,7 weeks after infection by indirect enzyme-linked immunosorbent assay.The expression levels of IFN-γ and IL-4 were detected by ELISA.The results showed that compared with the CA-treated group,the burden of adult and muscle larvae in mice immunized with BG+CA was significantly reduced(P＜0.0001),and BG+CA induced higher levels of IgG immune response and more expression of Dec-tin-1,IFN-γ,and IL-4.There were no significant differences in parasite load,IgG titer,IFN-γ andIL-4 expression between 2A11 inhibitor mice and CA treatment group(P＞0.05),targeting 2A11 antibody significantly reduced the immune response caused by BG.The results show that Dectin-1 is related to the protective immune response of CA triggered by BG against T.spiralis,and BG may be an effective immune adjuvant,providing a reference for the development of T.spiralis vac-cine.
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