ATF3在PHEV感染中的表达及作用
Expression and role of ATF3 in PHEV infection
陈雨竹 1王真真 1焦宇博 1胡锐杰 1王改丽 2石俊超 1贺文琦 1陆慧君 1高丰 1宋德光 1兰云刚1
作者信息
- 1. 吉林大学动物医学学院,吉林长春 130062
- 2. 吉林省畜牧兽医科学研究院,吉林长春 130062
- 折叠
摘要
为探究转录激活因子3(activating transcription factor 3,ATF3)在猪血凝性脑脊髓炎病毒(porcine hemaggluti-nating encephalomyelitis virus,PHEV)复制过程中的作用,本研究通过 Western blot、qRT-PCR和间接免疫荧光试验检测PHEV感染后ATF3表达水平及亚细胞定位变化,并在干扰ATF3后检测PHEV含量及相关炎性细胞因子变化.结果显示,PHEV感染后,ATF3的表达水平显著上调,同时ATF3从细胞浆转入细胞核中启动下游基因表达;而干扰ATF3后,细胞内炎性因子IFN-β,IFN-γ,IL-6,IL-1β转录水平下调,PHEV含量显著增加.结果表明,PHEV感染后上调ATF3可动态调节神经元中的炎性因子并对机体产生保护作用.
Abstract
In order to explore the function of activating transcription factor 3(ATF3)in the porcine hemagglutinating encephalomyelitis virus(PHEV)replication process,the current investigation was carried out using Western blot,qRT-PCR,and indirect immunofluorescence assays to identify changes in ATF3 expression level and subcellular localization following PHEV infection.Addition-ally,alterations in PHEV content and associated inflammatory cytokines were detected following ATF3 interference.According to the findings,ATF3 expression was dramatically increased follow-ing PHEV infection,and it translocated from the cytoplasm to the nucleus to trigger downstream gene expression.Interfering with ATF3 reduced the transcription levels of inflammatory factors IFN-β,IFN-γ,IL-6,and IL-1β,while increasing the PHEV content.The results indicated that up-regulation of ATF3 after PHEV infection could dynamically regulate inflammatory cytokines in neurons and had a protective effect on the body.
关键词
猪血凝性脑脊髓炎病毒/转录激活因子3/炎性因子/病毒复制Key words
PHEV/ATF3/inflammatory factors/virus replication引用本文复制引用
出版年
2024
NETL
NSTL
万方数据