首页|ATF3在PHEV感染中的表达及作用

ATF3在PHEV感染中的表达及作用

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为探究转录激活因子3(activating transcription factor 3,ATF3)在猪血凝性脑脊髓炎病毒(porcine hemaggluti-nating encephalomyelitis virus,PHEV)复制过程中的作用,本研究通过 Western blot、qRT-PCR和间接免疫荧光试验检测PHEV感染后ATF3表达水平及亚细胞定位变化,并在干扰ATF3后检测PHEV含量及相关炎性细胞因子变化.结果显示,PHEV感染后,ATF3的表达水平显著上调,同时ATF3从细胞浆转入细胞核中启动下游基因表达;而干扰ATF3后,细胞内炎性因子IFN-β,IFN-γ,IL-6,IL-1β转录水平下调,PHEV含量显著增加.结果表明,PHEV感染后上调ATF3可动态调节神经元中的炎性因子并对机体产生保护作用.
Expression and role of ATF3 in PHEV infection
In order to explore the function of activating transcription factor 3(ATF3)in the porcine hemagglutinating encephalomyelitis virus(PHEV)replication process,the current investigation was carried out using Western blot,qRT-PCR,and indirect immunofluorescence assays to identify changes in ATF3 expression level and subcellular localization following PHEV infection.Addition-ally,alterations in PHEV content and associated inflammatory cytokines were detected following ATF3 interference.According to the findings,ATF3 expression was dramatically increased follow-ing PHEV infection,and it translocated from the cytoplasm to the nucleus to trigger downstream gene expression.Interfering with ATF3 reduced the transcription levels of inflammatory factors IFN-β,IFN-γ,IL-6,and IL-1β,while increasing the PHEV content.The results indicated that up-regulation of ATF3 after PHEV infection could dynamically regulate inflammatory cytokines in neurons and had a protective effect on the body.

PHEVATF3inflammatory factorsvirus replication

陈雨竹、王真真、焦宇博、胡锐杰、王改丽、石俊超、贺文琦、陆慧君、高丰、宋德光、兰云刚

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吉林大学动物医学学院,吉林长春 130062

吉林省畜牧兽医科学研究院,吉林长春 130062

猪血凝性脑脊髓炎病毒 转录激活因子3 炎性因子 病毒复制

国家自然科学基金

32172804

2024

中国兽医学报
吉林大学

中国兽医学报

CSTPCD北大核心
影响因子:0.702
ISSN:1005-4545
年,卷(期):2024.44(4)
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