禽脑脊髓炎病毒VP1蛋白抗体间接ELISA检测方法的建立与临床应用
Establishment and clinical application of an indirect ELISA method for detecting VP1 protein antibody of avian encephalomyelitis virus
吕转平 1苏晓月 1唐思静 1王艳萍 1张多明2
作者信息
- 1. 新疆巴音郭楞职业技术学院,新疆库尔勒 841000
- 2. 新疆羌都畜牧科技有限公司,新疆巴音郭楞 841801
- 折叠
摘要
为建立1种禽脑脊髓炎病毒(avian encephalomyelitis virus,AEV)抗体检测方法,试验对AEV VP1蛋白进行截短表达,以表达蛋白为包被抗原建立检测AEV抗体的间接ELISA方法.结果显示,重组VP1蛋白以包涵体形式表达,大小为34 kDa;可与AEV阳性血清发生特异性反应;以VP1蛋白为包被抗原建立的间接ELISA方法检测ALV、IBDV、IBV、ILTV、NDV、AIV-H9阳性血清均为阴性;检测AEV抗体的最低检出效价为1:51 200;与IDEXX试剂盒的符合率为95.24％;检测新疆地区1 127份AEV疫苗免疫鸡血清样品和879份未免疫鸡血清样品的免疫抗体阳性率和感染抗体阳性率分别为88.91％和8.19％.结果表明,建立的间接ELISA方法特异、敏感、准确,可用于临床中免疫抗体和野毒感染抗体的检测和筛查.
Abstract
To establish a method for detecting antibodies against avian encephalomyelitis virus(AEV),the truncated VP1 protein of AEV was expressed and used as the coating antigen to estab-lish an indirect ELISA method for detecting AEV antibodies.The results showed that the recombi-nant VP1 protein was expressed in the form of an inclusion body with a size of 34 kDa and reacted specifically with AEV positive serum.The indirect ELISA method established was negative for de-tecting serum of ALV,IBDV,IBV,ILTV,NDV,AIV-H9.The lowest detectable titer for AEV an-tibodies was 1:51 200,with a coincidence rate of 95.24％with IDEXX reagent kit.The antibody positive rates were 88.91％and 8.19％for 1 127 chicken serum samples immunized with AEV vac-cine and 879 chicken serum samples with no vaccination,respectively in Xinjiang region.The indi-rect ELISA method established in this study was specific,sensitive,and accurate,and can be used for the detection and screening of AEV infection.
关键词
禽脑脊髓炎病毒/VP1蛋白/间接ELISA/临床应用Key words
avian encephalomyelitis virus/VP1 protein/indirect ELISA/clinical application引用本文复制引用
基金项目
新疆维吾尔自治区科技特派员基金(2022)(2022KF044)
出版年
2024
NETL
NSTL
万方数据