Establishment and clinical application of an indirect ELISA method for detecting VP1 protein antibody of avian encephalomyelitis virus
To establish a method for detecting antibodies against avian encephalomyelitis virus(AEV),the truncated VP1 protein of AEV was expressed and used as the coating antigen to estab-lish an indirect ELISA method for detecting AEV antibodies.The results showed that the recombi-nant VP1 protein was expressed in the form of an inclusion body with a size of 34 kDa and reacted specifically with AEV positive serum.The indirect ELISA method established was negative for de-tecting serum of ALV,IBDV,IBV,ILTV,NDV,AIV-H9.The lowest detectable titer for AEV an-tibodies was 1:51 200,with a coincidence rate of 95.24％with IDEXX reagent kit.The antibody positive rates were 88.91％and 8.19％for 1 127 chicken serum samples immunized with AEV vac-cine and 879 chicken serum samples with no vaccination,respectively in Xinjiang region.The indi-rect ELISA method established in this study was specific,sensitive,and accurate,and can be used for the detection and screening of AEV infection.
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