Pathogenicity of porcine sapelovirus in pigs of different ages
Porcine sapelovirus(PSV)is an enterovirus that can cause clinical symptoms such as pig-let diarrhea,pneumonia,reproductive disorders,and cerebral poliomyelitis.PSV can be detected in diarrhea or asymptomatic pigs throughout the world and cause huge economic losses to the pig in-dustry.In the previous study,our laboratory isolated a PSV strain from fecal samples of the diar-rhea piglets.To investigate its pathogenicity on piglets of different ages,the 3-day-old piglets and 2-month-old pigs were infected with the same dose of PSV.The results showed that 24 h after in-fection,3-day-old piglets showed significant clinical symptoms(watery diarrhea,tremors,and loss of appetite),while the 2-month-old pigs showed no significant clinical symptoms.After 3 d of virus infection,all pigs were dissected.The qRT-PCR results showed that the fecal virus content of 3-day-old piglets and 2-month-old pigs reached its peak at 48 h,and PSV had extensive tissue trop-ism in both 3-day-old and 2-month-old pigs.The results of histopathological examination showed that PSV infection could cause obvious intestinal pathological changes in 3-day-old piglets,inclu-ding atrophy and shedding of intestinal villus.While there was no obvious pathological change in the intestines of 2-month-old piglets.In summary,PSV can infect 3-day-old piglets and 2-month-old pigs and can replicate efficiently in vivo.However,it only causes obvious clinical symptoms in 3-day-old piglets,and there is no obvious pathogenicity to 2-month-old pigs.
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