猪萨佩罗病毒对不同日龄猪的致病性
Pathogenicity of porcine sapelovirus in pigs of different ages
司露露 1高俊龙 1张云飞 1仇聪蕊 1胡慧 2李成吾3
作者信息
- 1. 河南农业大学动物医学院,河南郑州 450046;动物病原与生物安全教育部重点实验室,河南郑州 450046
- 2. 河南农业大学动物医学院,河南郑州 450046;动物病原与生物安全教育部重点实验室,河南郑州 450046;河南省动物性食品安全重点实验室,河南郑州 450046
- 3. 河南农业大学动物医学院,河南郑州 450046
- 折叠
摘要
猪萨佩罗病毒(porcine sapelovirus,PSV)是可引起仔猪腹泻、肺炎、繁殖障碍和脑脊髓灰质炎等临床症状的肠道病毒,在腹泻猪和无症状的猪中均可检测到,在我国多地流行,给养猪业造成巨大的经济损失.前期本实验室从仔猪腹泻粪便样品中分离到1株PSV,为探究其对不同日龄猪的致病作用,本研究用相同剂量的PSV分别感染3日龄和2月龄猪.结果表明攻毒后24 h,3日龄猪出现明显的临床症状(水样腹泻、震颤和食欲不振等),2月龄猪无明显的临床症状.攻毒3 d后,对所有猪进行剖检,qRT-PCR结果表明,3日龄猪和2月龄猪粪便中病毒含量均在攻毒后48 h到达顶峰,且PSV在3日龄和2月龄猪体内均具有广泛的组织嗜性.组织病理学检查结果表明PSV感染3日龄猪只后,可引起明显的肠道病理变化,主要表现为肠绒毛萎缩、脱落等,而2月龄猪肠道无明显的病理变化.综上所述,PSV可感染3日龄和2月龄猪,且可以在体内高效复制,但仅引起3日龄猪发生明显的临床症状,对2月龄猪无明显的致病性.
Abstract
Porcine sapelovirus(PSV)is an enterovirus that can cause clinical symptoms such as pig-let diarrhea,pneumonia,reproductive disorders,and cerebral poliomyelitis.PSV can be detected in diarrhea or asymptomatic pigs throughout the world and cause huge economic losses to the pig in-dustry.In the previous study,our laboratory isolated a PSV strain from fecal samples of the diar-rhea piglets.To investigate its pathogenicity on piglets of different ages,the 3-day-old piglets and 2-month-old pigs were infected with the same dose of PSV.The results showed that 24 h after in-fection,3-day-old piglets showed significant clinical symptoms(watery diarrhea,tremors,and loss of appetite),while the 2-month-old pigs showed no significant clinical symptoms.After 3 d of virus infection,all pigs were dissected.The qRT-PCR results showed that the fecal virus content of 3-day-old piglets and 2-month-old pigs reached its peak at 48 h,and PSV had extensive tissue trop-ism in both 3-day-old and 2-month-old pigs.The results of histopathological examination showed that PSV infection could cause obvious intestinal pathological changes in 3-day-old piglets,inclu-ding atrophy and shedding of intestinal villus.While there was no obvious pathological change in the intestines of 2-month-old piglets.In summary,PSV can infect 3-day-old piglets and 2-month-old pigs and can replicate efficiently in vivo.However,it only causes obvious clinical symptoms in 3-day-old piglets,and there is no obvious pathogenicity to 2-month-old pigs.
关键词
猪萨佩罗病毒/致病性/荧光定量PCR/组织病理学Key words
PSV/pathogenicity/qRT-PCR/histopathology引用本文复制引用
基金项目
河南省重点研发专项(231111113100)
河南省自然科学基金交叉学科创新研究群体资助项目(232300421001)
出版年
2024
NETL
NSTL
万方数据