Isolation,identification and biological characterization of a strain GZPA426 of Wohlfahrtiimonas chitiniclastica
Wohlfahrtiimonas chitinicolatica(W.chitinicolatica)is a rare newly emerged zoonotic pathogen that can invade animal bodies and cause purulent diseases.In 2018,a pig farm in Guizhou sent two sick piglets for testing,which showed a typical H.parasuis mixed infection.Sterile collec-tion of diseased pig lung tissue was cultured,W.chitinicolatica was detected by 16S rRNA sequen-cing.To understand the pathogenic characteristics,the pathogen was isolated,purified and cultured.The pathogen was systematically identified according to culture characteristics of Gram stain,bio-chemical test,16S rRNA sequence analysis,and electron microscopy observation.Its drug suscepti-bility was determined through susceptibility testing;its pathogenicity was determined by the mouse pathogenicity test.The results showed that the nucleotide similarity between the isolate and W.chitinicolatica was 95.9％-97.6％,which is the closest genetic distance from the reference strain KBL006(NR_133893.1).It grows well on blood agar plates,LB agar medium,etc.,but does not grow on high-salt medium;biochemical identification results showed that the biochemical char-acteristics of the isolates were consistent with W.chitinicolatica.Electron microscopy showed that the length and width of the bacteria were 1.30 μm × 0.53 μm;the results of drug susceptibility test showed that the isolates were sensitive to cephalosporins,polymyxin B,certain penicillins and aminoglycosides,but resistant to tetracyclines,macrolides,and quinolones.The results of the mouse pathogenicity test showed that the isolated strain was lethal to mice.In this study,a strain of W.chitinicolatica sucis was successfully isolated,and its pathogenic biological characteristics were preliminarily studied,and the results provide an important reference for the pathogen detec-tion,prevention and control of W.chitinicolatica.
Wohlfahrtiimonas chitiniclasticaisolation and identificationsusceptibility testingmouse pathogenicity test
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