cpe localization of cpe+Clostridium perfringens isolates
Clostridium perfringens(C.perfringens)can cause food poisoning in humans and many non-foodborne gastrointestinal(GI)diseases.C.perfringens enterotoxin(CPE),whose encoding gene cpe locates on the chromosome is prone to cause human food poisoning,while C.perfringens with cpe locates on the plasmid is associated with non-foodborne human GI diseases.In this study,the localization of cpe of cpe+isolates were analyzed by PCR amplification,single-locus sequence typing(SLST)as well as the heat resistance of vegetative cells and spores.The duplex PCR results showed that only 600 bp bands were amplified from 29 cpe+isolates,IS1470,IS1470-like and IS1151 target bands were not detected.The conventional sod and sodFPF PCR detection as well as SLST analysis showed that cpe of the detected isolates may locate on chromosome.The heat resist-ance of vegetative cells and spores of strain 7-1 and 21-3 were higher than that of 42-2,it was spec-ulated that cpe of 7-1 and 21-3 locate on chromosomes while cpe of 42-2 locates on plasmid.In con-clusion,it provides materials and scientific basis for the diagnosis,prevention and control of food poisoning and non-foodborne diseases,as well as the correlation between cpe location and the dis-ease caused by C.perfringens.
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