Characterization of Clostridium perfringens type D from sheep sources in Shaanxi,Gansu and Ningxia
This study conducted toxin gene identification,drug resistance detection,disinfectant sus-ceptibility testing and genetic characterization analysis of 30 strains of Clostridium perfringen type D isolated from four sheep farms in Shaanxi,Gansu and Ningxia of China during the period of 2020 to 2022.Toxin gene identification showed that all strains carried the toxin genes cpa and etx,and did not carry other typing-related toxin genes.The drug resistance detection showed that the i-solates were more resistant to sulfisoxazole and cotrimoxazole,with the resistance rate of 30.00％and 63.33％,respectively;they were sensitive to penicillin,linezolid,clindamycin,doxycycline,en-rofloxacin and florfenicol,with the MIC values concentrated below 1 mg/L.The isolates were com-pletely killed after treatment with concentrations of neosporin,ethanol,84 disinfectant and concen-trated glutaraldehyde for 10 mins.After multilocus sequence typing(MLST)analysis,seven differ-ent housekeeping gene combinations were identified among the 30 strains of C.perfringens type D;the same housekeeping gene combinations and closer evolutionary relationships were often found between strains originating from the same field area and from the same province,and the same housekeeping gene combinations were found between some strains from Shaanxi and Gansu Provinces,which revealed the spread of C.perfringens type D within the sheep farms and between different provinces.In conclusion,this study revealed the geographical prevalence and transmission of C.perfringens type D to a certain extent at the genetic level,suggesting that the rational use of disinfectants and antibiotics should be strengthened in production to reduce the transmission and pathogenesis of C.perfringens type D.
Clostridium perfringens type Ddrug resistancedisinfectant resistancegenetic charac-teristics
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NSTL
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