橄榄苦苷对LPS诱导小鼠视网膜损伤的保护作用
Protective effect of oleuropein on LPS-induced retinal damage in mice
陈文东 1朱秀娟 1叶文斌 1王昱 1黄新异 2邸多隆 2徐永平3
作者信息
- 1. 陇南师范高等专科学校农林技术学院,甘肃成县 742500
- 2. 中国科学院兰州化学物理研究所 中国科学院西北特色植物资源化学重点实验室和甘肃省天然药物重点实验室,甘肃兰州 730000
- 3. 西北农林科技大学动物医学学院,陕西杨凌 712100
- 折叠
摘要
为研究橄榄苦苷(oleuropein,OP)对LPS诱导小鼠视网膜损伤的保护作用,将SPFKM小鼠随机分成5组,分别为LPS模型组(LPS)、对照组(Control)、OP高、中、低质量浓度(800、400、200 mg/kg)处理组,连续灌胃21 d,从第14天开始,各组小鼠灌胃30 min后,在异氟烷气体辅助麻醉下,小鼠腹腔注射2.0 mg/kg LPS,对照组小鼠腹腔注射2.0 mg/kg生理盐水,连续7 d,建模完成后,检测小鼠血清中超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性及白细胞介素-1 β(IL-1β)和肿瘤坏死因子-α(TNF-α)含量,采用免疫组化SP法检测甘油三脂脂肪酶(PNPLA2)在小鼠视网膜各层结构上的分布特点并观察视网膜组织形态学变化.结果显示,OP使小鼠血清中SOD、CAT和GHS-Px活性显著升高(P＜0.01),IL-1β和TNF-α含量下降(P＜0.05),视网膜中PNPLA2表达量升高(P＜0.05,P＜0.01);OP各剂量组均能提高SOD、CAT和GHS-Px活性,降低IL-1β和TNF-α含量,改善LPS对小鼠视网膜的病理损伤,提高视网膜中PNPLA2表达量,具有保护LPS诱导视网膜损伤的作用.
Abstract
To investigate the protective effect of oleuropein(OP)on LPS-induced retinal damage in mice.The mice were randomly divided into 5 groups:LPS model group(LPS),control group(Con-trol),high,medium,and low dose OP treatment groups(800,400,200 mg/kg),the OP was admin-istered to the mice via intragastric administration for 21 consecutive days,starting from the 14th day.After 30 min of intragastric administration under isoflurane-assisted anesthesia,experimental group mice were intraperitoneally injected with 2.0 mg/kg LPS and control group mice were injec-ted with the same dose of physiological saline for continuous 7 days,after modeling was comple-ted,the activities of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px)and the content of interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)in the serum were determined,immunohistochemical SP method was used to study the distribution characteristics of glycerol triglyceride lipase(PNPLA2)in the retina in mice,and observe the mor-phological changes of retinal tissue.The results showed that OP could significantly improve the ac-tivity of SOD,CAT and GSH-Px(P＜0.01),reduce the IL-1β and TNF-α content(P＜0.05)in se-rum,and increase expression of PNPLA2 in the retina(P＜0.05,P＜0.01).Oleuropein in all dose groups could improve the activities of SOD,CAT and GSH-Px,reduce the content of IL-1 β and TNF-α,improve the pathology of LPS-induced retinal damage,increase the expression of PNPLA2 in the retina.Therefore,it can be concluded that the OP has a protective effect against LPS-induced retinal damage.
关键词
橄榄苦苷/脂多糖/视网膜/小鼠Key words
oleuropein/lipopolysaccharide/retina/mice引用本文复制引用
基金项目
甘肃省科技重大专项(2021)(21ZD4NK045)
甘肃省重点研发计划(2022)(22YF7FK220)
甘肃省自然科学基金(2021)(21JR7RK912)
陇南师专校级研究中心项目(2023)(2023B-01)
陇南市科技计划(2020-ZD-06)
出版年
2024
NETL
NSTL
万方数据