四川省雷波县山羊和绵羊体表寄生蜱携带斑点热群立克次体与无形体分子流行病学调查
Molecular epidemiological survey on Anaplasmataceae and spotted fever group Rickettsia in ticks collected from sheep and goats in Leibo county of Sichuan Prov-ince,China
吉克日洪 1向阳 2陈虹汐 1袁东波 3尹念春 4郝力力1
作者信息
- 1. 西南民族大学畜牧兽医学院,四川成都 610041
- 2. 西南民族大学畜牧兽医学院,四川成都 610041;盐亭县高端人才服务中心,四川绵阳
- 3. 四川省动物疫病预防控制中心,四川成都 610041
- 4. 遂宁市农业农村局,四川遂宁 629000
- 折叠
摘要
旨在了解四川省雷波县山羊和绵羊源寄生蜱种类及其携带斑点热群立克次体(spotted fever group Rickettsia,SFGR)和无形体(Anaplasmataceae)的流行情况.采集山羊和绵羊源寄生蜱,先进行形态学初步鉴定,然后提取蜱总DNA,分别扩增蜱ITS-2、立克次体gltA和ompA与无形体16S rRNA和rpoB的部分基因片段,并对所有PCR阳性产物测序、比对并构建遗传进化树,以确定蜱种类及其感染SFGR和无形体的情况.共采集到353只山羊和绵羊体表寄生蜱,经鉴定均为长角血蜱(Haemaphysalis longicornis).SFGR总感染率为22.66％(80/353),仅检出1个种类R.raoultii,与西藏报道的银盾革蜱上分离的R.raoultii(JQ792163)亲缘关系最近.无形体总感染率为87.82％(310/353),共检出 6 种无形体和 2 种埃立克体,分别为 A.bovis、A.capra、A.mar ginale、Ana plasma cf.mar ginale、Candidatus A.mediterraneum、Ana plasma sp.、Ehrichia chaffeensis 和 Ehrichia sp.部分长角血蜱存在混合感染,混合感染率为7.37％(26/353).结果表明长角血蜱可能是当地的优势蜱种,SFGR和无形体的感染率较高,今后应加强上述蜱传病原的监测.
Abstract
To investigate tick species and prevalence of Anaplasmataceae and spotted fever group Rickettsia(SFGR)in ticks collected from sheep and goats in Leibo county,Sichuan Province.Ticks were preliminary identified by morphological characters and the genomic DNAs of ticks were ex-tracted.Molecular detection was performed by PCR targeting gene fragment of tick ITS-2,gltA and ompA genes of Rickettsia spp.,16S rRNA and rpoB genes of Anaplasma spp.and Ehrlichia spp.Positive PCR products were sequenced and phylogenetic trees were built based on these genes.The results showed that a total of 353 adult ticks collected from sheep and goats were identified as H.longicornis.The total prevalence of SFGR in H.longicornis was 22.66％(80/353)with only R.raoultii detected,which was closely related to R.raoultii(JQ792163)isolated from Dermacentor niveus in Tibet.The total prevalence of Anaplasmataceae was 87.82％(310/353)with six Ana-plasma species(A.bovis,A.capra,A.marginale,Anaplasma cf.marginale,Anaplasma sp.,Can-didatus A.mediterraneum)and two Ehrlichia species(E.chaffeensis and Ehrlichia sp.)detec-ted.Go-infection was found in some H.longicornis as high as 7.37％(26/353).The results indica-ted that H.longicornis may be the dominant tick species in Leibo county with high infections rates of Anaplasmataceae and SFGR.The close monitoring of the tick-borne pathogens should be strengthened in the future research work.
关键词
雷波县/蜱/斑点热群立克次体/无形体/分子检测Key words
Leibo county/tick/SFGR/Anaplasmataceae/molecular detection引用本文复制引用
基金项目
西南民族大学研究生创新型科研项目(ZD2022668)
出版年
2024
NETL
NSTL
万方数据