猴耳环提取物对黄羽肉鸡肠道健康和肠道菌群的影响
Effects of Pithecellobium Clypearia extract on intestinal health and microbial di-versity of yellow-feathered broilers
付新亮 1金少冰 1杨明炜 1叶志豪 2刘振东 2李冰心 1曹楠 1李婉雁 1许丹宁 1田允波 1黄运茂1
作者信息
- 1. 仲恺农业工程学院动物科技学院,广东广州 510225;广东省水禽健康养殖工程技术研究中心,广东广州 510225
- 2. 广东态合堂实业有限公司,广东惠州 516000
- 折叠
摘要
旨在探究饲粮中添加猴耳环提取物对黄羽肉鸡肠道黏膜组织结构、肠道炎性损伤及肠道菌群的影响.选用240只1日龄黄羽肉鸡随机分为A、B、C和D组,其中A、B和C组基础日粮分别添加0.5、1.0、2.0 g/kg的猴耳环提取物,D组为空白对照组,试验期为70 d.分别于试验第20、40和70天采集鸡十二指肠和空肠,测定各试验组十二指肠和空肠绒隐比、空肠组织炎性细胞因子和相关通路基因表达水平.并采集试验第70天各试验组盲肠内容物,采用16S rDNA测序分析微生物菌群结构.试验结果表明:饲粮中添加0.5和1.0 g/kg的猴耳环提取物可显著增加黄羽肉鸡十二指肠和空肠的绒隐比及空肠组织中紧密连接蛋白CLDN1和CLDN5的基因表达水平(P＜0.05),改善肠道黏膜组织结构和物理屏障功能;饲粮中添加猴耳环提取物可显著降低黄羽肉鸡空肠组织中IL-1β、IL-8和TNF-α等炎性因子及TLR4、MyD88和NF-κB等炎性通路的基因表达水平(P＜0.05),减少肠道组织的炎性损伤;饲粮中添加猴耳环提取物可显著增加黄羽肉鸡盲肠中副拟杆菌属和普雷沃菌属等有益菌群的丰度,并显著降低盲肠中变形菌门等有害菌群的丰度(P＜0.05),从而改善肠道菌群结构.综上,饲粮中添加猴耳环提取物能够显著改善黄羽肉鸡的肠道黏膜组织结构和物理屏障功能,减少肠道组织炎性损伤,并改善盲肠微生物菌群结构,促进肠道健康,为将猴耳环开发为安全、有效的饲料添加剂提供科学支撑和理论依据.
Abstract
To investigate the effects of Pithecellobium clypearia extract on the tissue structure,in-flammatory lesions as well as microbial diversity in the intestinal of yellow-feathered broilers.2401-day-old yellow-feathered broilers were randomly divided into four groups(groups A,B,C and D),groups A,B and C were supplemented with Pithecellobium clypearia extract in basal diets with concentrations of 0.5,1.0 and 2.0 g/kg,respectively.Group D served as the control group without adding Pithecellobium clypearia extract in diets,and the full trial period lasted for 70 d.Duodenum and jejunum samples were collected on the 20th,40th and 70th days of the test,the vil-lous/crypt ratio of duodenum and jejunum were calculated,and the mRNA expression level of in-flammatory cytokine as well as related pathways were detected in each group,respectively.In addition,the contents of cecum were collected at 70 th day of the experiment and the microbial di-versity in cecum were also analysed by 16S rDNA sequencing.The results showed that adding 0.5 and 1.0 g/kg of Pithecellobium clypearia extract in the diet could significantly increase the veloci-ty height/crypt depth ratio of duodenum and jejunum(P＜0.05)compared to control group,as well as the mRNA expression level of tight junction protein(CLDN1 and CLDN5)in jejunum,which further improved the structure of mucous of intestinal.Pithecellobium clypearia extract could significantly(P＜0.05)decrease the mRNA expression level of inflammatory cytokine inclu-ding IL-1β,IL-8 and TNF-α,as well as the related pathway genes such as TLR4,MyD88 and NF-κB in jejunum,thus reduced the inflammatory lesions in intestinal.Pithecellobium clypearia ex-tract also could significantly increase the abundance of beneficial microbial such as Parabacteroide and Prevotellaceae,while significantly decrease the abundance of pathogenic microbial such as Proteobacteria in cecum(P＜0.05)and improve the microbial diversity in intestinal.In summary,Pithecellobium clypearia extract could improve the structure of intestinal tissue and the gut barri-er function,as well as the microbial diversity in cecum,and also decrease the inflammatory lesions in jejunum,which is helpful to the intestinal health for yellow-feathered broilers.The present study provides scientific basis for the development of Pithecellobium clypearia as a safe feed additive in the future.
关键词
猴耳环/黄羽肉鸡/肠道健康/绒隐比/炎性损伤/肠道菌群Key words
Pithecellobiumclypearia/yellow-feathered broilers/intestinal health/villous/crypt rati-o/inflammatory lesions/microbial diversity引用本文复制引用
基金项目
广东省基础与应用基础研究基金区域联合基金-青年基金(2021A1515111167)
广州市科技计划基础与应用基础研究项目(202102021235)
出版年
2024
NETL
NSTL
万方数据