室旁核VGlut2神经元投射通路
Projection pathway of VGlut2 neurons from paraventricular nucleus
王星 1刘昊东 1李鹏辉 1李嘉成 1樊奇 1闫睿 1贺炀 1张明 1周鑫 1杜晨光2
作者信息
- 1. 内蒙古农业大学兽医学院,内蒙古呼和浩特 010018;内蒙古自治区基础兽医学重点实验室,内蒙古呼和浩特 010018
- 2. 内蒙古农业大学兽医学院,内蒙古呼和浩特 010018;内蒙古自治区基础兽医学重点实验室,内蒙古呼和浩特 010018;内蒙古农业大学职业技术学院,内蒙古包头 014109
- 折叠
摘要
囊泡型谷氨酸转运蛋白2(VGlut2)作为兴奋性神经递质表达于下丘脑室旁核(PVNVGlut2),调节摄食和能量代谢,对维持机体稳态具有重要作用.但尚不清楚PVNVGlut2神经元上、下游投射网络,阻碍了解析谷氨酸能神经元环路功能.因此,本研究采用脑立体定位注射技术,将顺行和逆行示踪病毒注射到VGlut2小鼠的PVN,寻找PVNVGlut2神经元的输入和输出核团.顺行示踪结果表明,PVNVGlut2神经元投射到下游内侧杏仁核(MeAD)和弓状核(ARC).逆行示踪结果表明.PVNVGlut2接受前置核(Pr)、桥脑网状被盖核(RtTg)、舌下神经核(12N)输入.此外,在PVN发现VGlut2与神经元型一氧化氮合酶(nNOS)神经元共表达.通过病毒追踪工具解析PVNVGlut2神经元的解剖学网络,为后续研究剖析PVNVGlut2功能调控奠定基础.
Abstract
Vesicular glutamate transporter 2(VGlut2)is expressed in the PVN of the hypothalamic paraventricular nucleus(PVNVG1ut2)as an excitatory neurotransmitter,which regulates food intake and energy metabolism and plays an important role in maintaining homeostasis.However,it is not clear that the upstream and downstream projection network of PVNVGut2 neurons hinders the anal-ysis of glutamatergic neuron circuit function.Anterograde and retrograde tracer viruses were injec-ted into the PVN of VGlut2 mice by stereotactic brain injection technique to find the input and output nuclei of PVNVGlut2 neurons.Anterograde tracing results showed that PVNVGlut2 neurons pro-jected to the downstream medial amygdala(MeAD)and arcuate nucleus(ARC).Retrograde trac-ing results showed that PVNVGlut2 received input from the prefrontal nucleus(Pr),the reticular tegmental nucleus(RtTg),and the hypoglossal nucleus(12N).In addition,VGlut2 was found to be co-expressed with neuronal nitric oxide synthase(nNOS)neurons in the PVN.The anatomical net-work of PVNVG1ut2 neurons was analyzed by virus tracking tool,which laid the anatomical founda-tion for further study on the functional regulation of PVNVGlut2.
关键词
下丘脑室旁核/谷氨酸能神经元/神经投射/能量代谢/脑立体定位注射/小鼠Key words
hypothalamic paraventricular nucleus/glutamatergic neurons/nerve projection/energy metabolism/stereotactic brain injection/mice引用本文复制引用
基金项目
国家自然科学基金(32060204)
内蒙古科技攻关计划(2021GG0199)
内蒙古科技攻关计划(2022YFHH0015)
内蒙古教育厅高等学校科研项目(NJZY22535)
内蒙古教育厅高等学校科研项目(NJZY23120)
内蒙古自治区直属高校基本科研业务费专项(BR231507)
学院草食家畜优异种质资源科技创新团队项目(TDY202302)
出版年
2024
NETL
NSTL
万方数据