基于Toll样信号通路探讨FSE对大鼠乳腺炎的治疗作用
Exploring therapeutic effects of Forsythia suspensa extracts on rat mastitis based on Toll-like signaling pathway
成晓丽 1曹璐 1王桃 1姚丹 1张勇1
作者信息
- 1. 甘肃农业大学动物医学院,甘肃兰州 730070;甘肃省动物生殖生理及繁殖调控重点试验室,甘肃兰州 730070
- 折叠
摘要
旨在探讨连翘提取物(Forsythia suspensa extracts,FSE)对金黄色葡萄球菌诱导大鼠乳腺上皮细胞炎症损伤的保护机制.选择哺乳期大鼠50只,随机分为5组,每组10只:空白对照组(第4对乳腺注射生理盐水),模型组(第4对乳腺注射金黄色葡萄球菌菌液各100 μL),高剂量组(第4对乳腺注射菌液24 h后,1～7 d灌胃FSE 3.750 g/(kg·d)),中剂量组(第4对乳腺注射菌液24 h后,1～7 d灌胃FSE 1.875 g/(kg·d)),低剂量组(第4对乳腺注射菌液24 h后,1～7 d灌胃FSE 0.935 g/(kg·d)),末次给药24 h后采集血样后处死.结果显示,模型组乳腺组织严重受损,乳腺组织中检测到核因子κB抑制物激酶β(IKKβ)、髓样分化因子88(MyD88)、核因子κB(NF-κB)、Toll样受体4(TLR4)mRNA表达量显著升高(P＜0.05).FSE中、高剂量与模型组相比,乳腺组织中IKKβ、MyD88、NF-KB、TLR4蛋白的相对表达量显著降低(P＜0.05).因此,FSE可能通过抑制Toll样信号通路改善金黄色葡萄球菌诱导的大鼠乳腺上皮细胞炎症反应,降低下游通路蛋白IKKβ、MyD88、NF-KB、TLR4的表达,从而发挥抗炎作用.
Abstract
The aim of the present study was to investigate the protective mechanism of Forsythia suspensa extracts(FSE)on S.aureus-induced inflammatory injury of rat mammary epithelial cells.Fifty lactating rats were selected and randomly divided into five groups with 10 rats each:the blank control group(the fourth pair of mammary glands were injected with saline),the model group(the fourth pair of mammary glands were injected with 100 pL of S.aureus bacterial solution),the high-dose group(the fourth pair of mammary glands were injected with S.aureus bacterial solu-tion for 24 h,and then the rats were administered by gavage of 3.750 g/(kg·d)FSE for 1-7 d),the medium-dose group(24 h after the fourth pair of mammary injections,the rats were adminis-tered by gavage 1.875 g/(kg·d)FSE for 1-7 d),and in the low-dose group(24 h after the fourth pair of mammary injections,the rats were administered by gavage 0.935 g/(kg·d)FSE for 1-7 d),blood samples were collected 24 h after the last administration of the drug and then the rats were executed.The results showed that the mammary tissue of the model group was severely dam-aged,and the mRNA expression of IKKβ,MyD88,NF-κB,and TLR4 was significantly increased in the mammary tissue(P＜0.05).The relative expression of IKKβ,MyD88,NF-κB,and TLR4 pro-teins in the mammary tissue of the model group was significantly decreased(P＜0.05)by medium and high doses of FSE compared with the model group.Therefore,FSE may play an anti-inflammatory role by inhibiting the Toll-like signaling pathway to improve S.aureus-induced inflammatory re-sponse in rat mammary epithelial cells and decrease the expression of the downstream pathway proteins IKKβ,MyD88,NF-κB,and TLR4.
关键词
连翘/金黄色葡萄球菌/乳腺炎/TLR4/MyD88/大鼠Key words
Forsythia suspensa/Staphylococcus aureus/mastitis/TLR4/MyD88/rat引用本文复制引用
基金项目
甘肃省动物生殖生理及繁殖调控重点实验室建设项目(20JR10RA563)
宁夏回族自治区重点研发资助项目(2019BBF02027)
出版年
2024
NETL
NSTL
万方数据