饲粮中添加凉茶渣对白羽肉鸡生长性能、血清生化及肠道免疫指标的影响
Effects of herbal tea residue supplement on growth performance,serum biochemi-cal and intestinal immune indexes in white feather broilers
郭玥 1欧阳坤 1刘彬 1陈碧兰 1罗君谊 1孙加节 1习欠云 1张永亮 1陈婷1
作者信息
- 1. 华南农业大学动物科学学院广东省动物营养调控重点实验室/国家生猪种业工程中心/猪禽种业全国重点实验室,广东广州 510642
- 折叠
摘要
旨在研究饲粮中添加王老吉凉茶渣对白羽肉鸡生长性能、血清指标和肠道免疫指标的影响.选取1日龄体质量相近、健康状态良好的817白羽肉杂鸡280只,随机分为4组,每组5个重复,每个重复14只.Ⅰ组为对照组,饲喂基础饲粮,Ⅱ、Ⅲ、Ⅳ组分别在基础饲粮中添加2％、4％、6％的凉茶渣粉,试验期49 d,分1～21和22～49日龄2个阶段.结果显示:1)与Ⅰ组相比,Ⅲ组21、49日龄体质量均显著增加(P＜0.05),22～49和1～49日龄的平均日采食量均显著上升(P＜0.05),1～21、22～49和1～49日龄的平均日增重均显著增加(P＜0.05).2)与Ⅰ组相比,21日龄时,Ⅱ组血清总胆固醇(T-CHO)显著降低(P＜0.05),Ⅱ、Ⅲ和Ⅳ组甘油三酯(TG)含量均显著减少(P＜0.05),Ⅳ组低密度脂蛋白胆固醇(LDL-C)含量和谷草转氨酶(AST)活性均降低(P＜0.05);49日龄时,Ⅱ组AST活性、Ⅲ组T-CHO含量和Ⅳ组LDL-C含量均降低(P＜0.05),Ⅲ和Ⅳ组高密度脂蛋白胆固醇(HDL-C)含量均升高(P＜0.05),谷丙转氨酶(ALT)活性均降低(P＜0.05).3)在十二指肠中,与Ⅰ组相比,21日龄时,Ⅱ组的TNF-a表达显著降低(P＜0.05),49日龄时,Ⅱ组的IL-10表达升高(P＜0.05),Ⅲ、Ⅳ组的IL-2表达显著降低(P＜0.05);在空肠中,与Ⅰ组相比,49日龄时,Ⅱ组IL-10的表达上升(P＜0.05),Ⅲ、Ⅳ组的IL-2表达显著降低(P＜0.05).结果表明,饲粮中添加凉茶渣在不影响白羽肉鸡正常生长性能的基础上,可调节血脂水平,同时增强肠道免疫.在本试验条件下,饲粮中添加2％～4％的凉茶渣效果较好.
Abstract
This experiment aims to investigate the effects of a dietary supplement of herbal tea resi-due on white feather broilers in the prospectives of growth performance,serum indexes and intesti-nal immune indexes.A total of 280 1-day-old white feather broilers with similar body mass and good health were randomly divided into 4 groups with 5 replicates per group and 14 birds per repli-cate.Group Ⅰ(control group)was fed a basal diet.Groups Ⅱ,Ⅲ and Ⅳ were fed a diet supple-mented with 2％,4％and 6％herbal tea residue powder,respectively.The feeding lasted for 49 days,and was divided into 2 phases from 1 to 21 days of age and 22 to 49 days of age.Blood,tissue and mucosa of the duodenum and jejunum were collected on 21 d and 49 d.Total cholesterol(T-CHO),triglyceride(TG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C)and the activities of aspartate aminotransferase(AST)and alanine amin-otransferase(ALT)in the serum were examined.The villus height and crypt depth of the jejunum were observed and measured by morphology.The mRNA expression levels of intestinal interleukin-1β(IL-1β),interleukin-2(IL-2),interleukin-4(IL-4),interleukin-10(IL-10)and tumor necrosis factor-α(TNF-α)were determined by qPCR.The results showed that compared with group Ⅰ,the mass of group Ⅲ on 21 d and 49 d significantly increased(P＜0.05).The average daily feed intake of 22-49 d and 1-49 d significantly increased(P＜0.05).The average daily mass gain of 1-21 d,22-49 d and 1-49 d significantly increased(P＜0.05).In the serum of 21 d,compared with group Ⅰ,T-CHO in group Ⅱ significantly decreased(P＜0.05).TG in groups Ⅱ,m and Ⅳ significantly de-creased(P＜0.05).LDL-C and AST activity in group Ⅳ significantly decreased(P＜0.05).In the serum of 49 d,T-CHO in group Ⅲ,LDL-C in group Ⅳ,HDL-C of groups Ⅲ and Ⅳ significantly increased(P＜0.05),and the ALT activity of these two groups decreased(P＜0.05).AST activity in group Ⅱ significantly decreased(P＜0.05).In the duodenum of 21 d,compared with group Ⅰ,the expressions of TNF-α in group Ⅱ was significantly decreased(P＜0.05).In the duodenum of 49 d,the expression of IL-10 in group Ⅱ was significantly increased(P＜0.05),and the expression of IL-2 in groups Ⅲ and Ⅳ was significantly decreased(P＜0.05).In the jejunum of 49 d,the ex-pression of IL-10 in group Ⅱ was increased(P＜0.05),and the expression of IL-2 in groups Ⅲand Ⅳ was significantly decreased(P＜0.05).In conclusion,dietary herbal tea residue can regulate circulating lipid level and enhance intestinal immunity of white feather broilers without affecting their normal growth performance.This experiment also suggested that the a 2％-4％supplementa-tion of herbal tea residues to white feather broilers was of good effect.
关键词
凉茶渣/白羽肉鸡/生长性能/血清指标/肠道免疫指标Key words
herbal tea residue/white feather broiler/growth performance/serum index/intestinal immune index引用本文复制引用
基金项目
国家重点研发计划(十四五)(2022YFD1300401)
猪禽种业全国重点实验室开放基金(2023GZ22)
国家留学基金面上项目(202008440191)
广东省自然科学基金面上项目(2021A1515011310)
广东省自然科学基金(2019A1515011734)
出版年
2024
NETL
NSTL
万方数据