猪瘟病毒跨细胞膜内化分子机制及猪瘟的防控研究进展
Research progress on molecular mechanism of transmembrane internalization of CSFV and prevention and control of CSF
邹宏 1程鹏 2郭建华 3罗干 2黄石磊4
作者信息
- 1. 重庆三峡职业学院动物科技学院,重庆万州 404100;西南大学动物医学院,重庆荣昌 402460
- 2. 重庆市万州区畜牧产业发展中心,重庆万州 404100
- 3. 西南大学动物医学院,重庆荣昌 402460
- 4. 重庆三峡职业学院动物科技学院,重庆万州 404100
- 折叠
摘要
猪瘟(CSF)是由猪瘟病毒(CSFV)引起的一种猪的临床上以高热稽留和广泛性出血为主要特征的传染病,是WOAH通报性疫病.CSFV作为一种单链RNA囊膜病毒,在适应外界环境压力的过程中,已经演变出一套吸附和侵入细胞的策略,但具体的分子机制尚不完全清楚.研究表明,CSFV主要通过网格蛋白介导的跨细胞膜内化,在胞质内以胞内体的形式存在,在一定条件下胞内体囊膜融合释放基因组进行病毒增殖.此间CSFV依赖与宿主蛋白发生相互作用,抑制宿主干扰素的合成分泌,调控宿主细胞凋亡、自噬、焦亡和炎症反应等生命活动,逃避宿主的天然免疫,从而促进病毒在宿主体内进一步复制,但具体的机制有待进一步研究.本文就CSFV跨细胞膜内化的分子机制和CSF的防控难题展开概述,以期为CSF的净化提供理论依据.
Abstract
Classical swine fever(CSF),caused by classical swine fever virus(CSFV),is a severe in-fectious disease characterized by high fever and extensive bleeding,which is listed as a mandatory report disease by WOAH.As a single-stranded RNA envelope virus,CSFV has evolved a strategy for attachment and entry to the cell in the process of adapting to external environmental pressure.H owever,the underlying molecular mechanism remains largely unknown.Studies have shown that CSFV is mainly internalized through clathrin-mediated cross-membrane internalization and exists in the form of endosomes in the cytoplasm.Under certain conditions,the endosomal membrane fu-sion releases the genome for viral proliferation.At the same time,CSFV also depends on the inter-action with host proteins to inhibit the synthesis and secretion of host interferon,regulate host cell apoptosis,autophagy,pyroptosis and inflammatory response,and other life activities to evade the host's natural immunity,thus promoting the further replication of the virus in the host.However,the underlying specific mechanism needs further studying.Here,we summarize the molecular mechanism of CSFV internalization across cell membranes and the challenges of CSF prevention and control,with a view to providing theoretical assistance for CSF purification.
关键词
猪瘟病毒/跨细胞膜内化/分子机制Key words
classical swine fever virus/transcellular membrane internalization/molecular mechanism引用本文复制引用
基金项目
重庆市教委科学技术研究计划(KJQN202203513)
重庆三峡职业学院重点科研项目(cqsx2021001)
出版年
2024
NETL
NSTL
万方数据