摘要
旨在探讨当归多糖(Chinese angelica polysaccharide,CAP)硒化前后对腹腔巨噬细胞功能的影响.试验将当归多糖和硒化当归多糖分别作用于小鼠腹腔巨噬细胞,用流式细胞术检测巨噬细胞的吞噬活性以及细胞表型F4/80、MHC-Ⅱ、CD80和CD86的表达量,并用ELISA检测巨噬细胞上清液中IL-6、IL-10、NO、MIP-1α和TNF-α含量变化.试验显示:sCAP质量浓度在1.96 mg/L时,腹腔巨噬细胞的吞噬活性显著高于其他各组(P＜0.05);F4/80、MHC-Ⅱ、CD80和CD86的表达量显著高于CAP组和细胞对照组(P＜0.05);质量浓度为7.81～1.96 mg/L时,巨噬细胞上清液中IL-6、IL-10、NO、MIP-1α的含量显著高于其他各组(P＜0.05).结果表明,sCAP对腹腔巨噬细胞免疫功能有增强作用,且以质量浓度1.96 mg/L最佳.
Abstract
The aim of this experiment was to investigate the effects of Chinese angelica polysaccha-ride(CAP)on peritoneal macrophage function before and after selenization.In the experiment,CAP and selenized Chinese angelica polysaccharide(sCAP)were applied to mouse peritoneal mac-rophages,and the phagocytic activity of macrophages as well as the expression of the cellular phe-notypes F4/80,MHC-Ⅱ,CD80 and CD86 were detected by flow cytometry,and the changes of IL-6,IL-10,NO,MIP-1α,and TNF-α content in the supernatant of macrophage were detected by ELISA.The results showed that the phagocytic activity of peritoneal macrophages was significantly higher in the sCAP mass concentration of 1.96 mg/L than in the other groups(P＜0.05);and the expression of F4/80,MHC-Ⅱ,CD80 and CD86 were significantly higher in the sCAP mass concen-tration of 1.96 mg/L than in the CAP group and cell control group(P＜0.05);the contents of IL-6,IL-10,NO,and MIP-1α in the supernatant of macrophages were significantly higher than those in other groups when the mass concentration of sCAP ranged from 7.81 to 1.96 mg/L(P＜0.05).The results showed that sCAP had an enhancing effect on the immunomodulatory function of ab-dominal macrophages and was optimal at a mass concentration of 1.96 mg/L.
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