摘要
采用肌肉注射pVAX1-SP-GHRH(1～44)表达质粒后电刺激处理,确定其对小鼠生长、妊娠母猪生产性能影响.将150只4周龄C57 BL/6雌性小鼠随机分成6组,每组5个重复.肌肉单次注射后电刺激处理,对照组注射空质粒(80 μg/kg),处理组分别注射20、40、80、120、160 μg/kg pVAX1-SP-GHRH(1～44)质粒.再选取20头健康的妊娠母猪随机分成2组,每组10头母猪.对妊娠后期的母猪半膜肌进行单次注射后电刺激处理.对照组注射生理盐水,质粒组注射2 mg pVAX1-SP-GHRH(1～44)表达质粒.分别在注射后0、7、14、21、28 d测定小鼠体质量、采食量,采眼球血、分离血清测定血清GHRH、IGF-1水平.妊娠母猪分别在注射后0、14、28、42 d进行尾静脉采血,分离血清,测定血清GHRH、IGF-1水平,在第14天,记录仔猪初生质量、胎盘质量、产子数、健仔数、弱仔数、畸形仔数、死胎数和木乃伊数.小鼠试验结果表明,肌肉注射pVAX1-SP-GHRH(1～44)质粒并电刺激后,能够促进小鼠的采食和提高增重(P＜0.05),能够显著提高小鼠血清GHRH、IGF-1水平(P＜0.05),且作用能够从注射后维持到21 d.妊娠母猪试验结果表明,质粒组妊娠母猪的仔猪平均初生质量极显著升高(P＜0.01)、胎盘质量显著升高(P＜0.05),质粒组母猪GHRH、IGF-1浓度极显著上升(P＜0.01).本研究结果表明,肌肉注射pVAX1-SP-GHRH(1～44)表达质粒并电刺激处理后能够促进小鼠的采食和提高增重,同时还能显著提高妊娠母猪仔猪初生质量、胎盘质量.
Abstract
The study adopted muscle injection of pVAX1-SP-GHRH(1-44)expression plasmid and electrostimulation to determine its effects on mouse growth and sow production performance.One hundred and fifty four-week-old C57 BL/6 female mice were randomly divided into 6 groups of 5 replicates each.Muscle single-injection followed by electrostimulation was performed.The con-trol group received an empty plasmid injection(80 μg/kg),while the treatment groups received pVAX1-SP-GHRH(1-44)plasmid injections(20,40,80,120,160 μg/kg).Twenty healthy preg-nant sows were randomly divided into 2 groups,each with 10 sows.Electrostimulation treatment was applied to the semimembranosus muscle of the pregnant sows after a single injection.The con-trol group received physiological saline injection,while the plasmid group received a 2 mg pVAX1-SP-GHRH(1-44)expression plasmid injection.Mouse weight,feed intake,and serum GHRH and IGF-1 levels were measured at days 0,7,14,21,and 28 after injection.Pregnant sows were bled via the tail vein at days 0,14,28,and 42 after injection,and their serum was separated to measure serum GHRH and IGF-1 levels.The birth weight,placental weight,number of piglets born,number of healthy piglets,number of weak piglets,number of deformed piglets,number of stillborn piglets,and number of mummified piglets were recorded at day 14.The mouse study re-sults showed that muscle injection of pVAX1-SP-GHRH(1-44)plasmid followed by electrostim-ulation could promote mouse feeding and increase weight gain(P＜0.05),significantly increase mouse serum GHRH and IGF-1 levels(P＜0.05),and maintain its effects until day 21.The results of the pregnant sow study showed that the average birth weight of the piglets in the plasmid group was significantly increased(P＜0.01),and the placenta weight was significantly increased(P＜0.05).The serum GHRH and IGF-1 concentrations in the plasmid group sows were significantly increased(P＜0.01).The study results showed that muscle injection of pVAX1-SP-GHRH(1-44)expression plasmid followed by electrostimulation could promote mouse feeding and increase weight gain,and also significantly improve the average birth weight and placental weight of the piglets in pregnant sows.
维普
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