摘要
利用MRS-CaCO3固体平板培养法从兔肠道内容物中分离并培养乳酸菌,通过形态学观察、革兰染色、生理生化特征观察、16S rDNA序列分析和ERIC-PCR分析进行鉴定,对符合乳酸菌特性的菌株进行生物学特性、抗逆性能、体外黏附性能、体内定植能力以及安全性等方面的研究.结果显示,共分离得到4株兔源罗伊乳杆菌;4株兔源罗伊乳杆菌均具有乳酸菌的典型生物学特征,对胃肠道内常见的病原菌具有一定的抑制作用,其发挥抑菌作用的主要物质为细菌素;对氯霉素、利福平及红霉素较敏感,对胃肠道环境以及高温具有一定的耐受能力;可在家兔体内成功定植且安全.本试验为开发预防和治疗兔肠道疾病的乳酸菌制剂奠定了基础.
Abstract
Lactic acid bacteria(LAB)were isolated and cultivated from the intestinal contents of rabbits by MRS-CaCO3 solid medium.Identification was achieved through morphological observa-tion,Gram staining,physiological and biochemical characterisation,16S rDNA sequence analysis,and ERIC-PCR analysis.Strains displaying typical Lactobacilli characteristics were exanimated for their biological characteristics,resistance properties,adherence capacity in vitro,colonization abili-ty in vivo,and safety profile.In this study,a total of four strains of Lactobacillus reuteri were iso-lated from rabbits,all of which exhibited typical biological characteristics of LAB.These strains demonstrated inhibitory effects on common pathogenic bacteria in the gastrointestinal tract,with the primary inhibitory substance being bacteriocin.Furthermore,they showed sensitivity to chlor-amphenicol,rifampicin,and erythromycin,and displayed a degree of tolerance to gastrointestinal conditions and high temperature.These stains were capable of successful colonization in rabbits with a higher degree of safety.This study lays a foundation for the development of LAB prepara-tions for the prevention and treatment of rabbit intestinal diseases.
基金项目
国家"十四五"重点研发计划资助项目(2022YFD1800800)
黑龙江省自然科学基金资助项目(YQ2021C020)
维普
万方数据