通过 Western blot、ELISA方法检测板蓝根多糖(Radix isatidis polysaccharide,IRPS)对猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)感染的 3D4/21/CD163 细胞 TLR3/TRIF 天然免疫途径和Ⅰ型干扰素分泌的影响,并使用TLR3激动剂Poly(I∶C)处理进一步验证IRPS对PRRSV感染引起的免疫抑制的影响.结果显示,PRRSV感染18、24 h时TLR3、TRIF、IRF3、IRF7蛋白水平及Ⅰ型干扰素分泌均显著下调,同时IRPS对这一过程具有显著的抑制作用;TLR3激动剂Poly(I∶C)能够缓解PRRSV感染引起的TRIF、IRF3、IRF7蛋白水平下调和IRF3、IRF7磷酸化水平降低,且IRPS与Poly(I∶C)具有协同作用.结果表明,IRPS能够通过TLR3/TRIF途径缓解PRRSV感染引起的免疫抑制.
Abstract
The effect of Radix isatidis polysaccharide(IRPS)on TLR3/TRIF innate immune path-way and type Ⅰ interferon secretion in 3D4/21/CD163 cells infected by porcine reproductive and re-spiratory syndrome virus(PRRSV)was tested by Western blot and ELISA;moreover,the effect of IRPS on the immunosuppression infected by PRRSV was further verified with the TLR3 agonist poly(I∶C).The results showed that the protein levels of TLR3,TRIF,IRF3,IRF7 and type Ⅰ in-terferon secretion were significantly decreased at 18,24 h of PRRSV infection,while IRPS signifi-cantly inhibited this process;poly(I∶C)alleviated the protein levels of TRIF,IRF3 and IRF7 as well as the phosphorylation levels of IRF3 and IRF7 infected by PRRSV;at the same time,IRPS is synergistic with poly(I∶C).The results indicate that IRPS is able to alleviate immunosuppression caused by PRRSV infection via the TLR3/TRIF pathway.
维普
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