中国荷斯坦奶牛ACOX1基因多态性及其与乳品质性状的相关分析

Genetic polymorphism of ACOX1 gene and its correlation with milk quality traits in China Holstein dairy cows

郝育嵘 ¹赵志辉 ¹游涛 ¹于海滨 ¹李光辉 ²姜平 ¹林紫薇¹

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作者信息

1. 广东海洋大学滨海农业学院,广东湛江 524088
2. 吉林大学动物科学学院,吉林长春 130062
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摘要

采用PCR扩增技术和直接测序法对83头中国荷斯坦奶牛群体中酰基辅酶A氧化酶1(acyl-CoA oxidase 1,ACOX1)基因的SNP位点进行检测;使用SPSS 25.0软件对ACOX1基因的遗传多态性位点基因型与中国荷斯坦奶牛乳品质性状进行关联分析.结果显示,在ACOX1基因的第3个内含子中发现了 1个SNP位点即I3-2 267 G→C,属于中度多态,并在群体中处于哈代温伯格平衡;关联分析发现,该SNP位点与奶牛乳体细胞含量和校正奶量显著相关.结果表明,ACOX1基因I3-2 267 G→C位点可作为中国荷斯坦奶牛乳品质性状的分子标志物,为中国荷斯坦奶牛品质性状研究提供参考.

Abstract

The SNP loci of ACOX1 gene in 83 China Holstein cows were detected by PCR amplifica-tion and direct sequencing,and the association between the genetic polymorphism loci of ACOX 1 gene and milk quality traits of China Holstein cows was analyzed by SPSS 25.0 software.The re-sults showed that a SNP locus I3-2 267 G→C was found in the third intron of ACOX1 gene,it was moderately polymorphic and in Hardy Weinberg equilibrium in the population.By correlation anal-ysis,it was found that the SNP locus was significantly related to the somatic cell content and cor-rected milk quantity of dairy cows.The I3-2 267 G→C locus of ACOX1 gene can be used as a mo-lecular marker of quality traits of Holstein cows in China,and provide reference for the study of quality traits of Holstein cows in China.

关键词

中国荷斯坦奶牛/ACOX1基因/乳品质/SNPs

Key words

China Holstein dairy cows/ACOX1/milk quality/single nucleotide polymorphism

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基金项目

国家自然科学基金资助项目(32002165)

国家自然科学基金资助项目(32072717)

广东省自然科学基金资助项目(2021A515010867)

广东海洋大学科研启动经费资助项目(R20060)

广东海洋大学2021年校级教育教学改革项目(010201052106)

出版年

2024

中国兽医学报

吉林大学

中国兽医学报

CSTPCD北大核心

影响因子：0.702

ISSN：1005-4545

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