为探讨核苷酸结合寡聚化结构域受体家族成员X1(nucleotide-binding domain and leucine-rich-repeat-containing family memberX1,NLRX1)在新城疫病毒(Newcastle disease virus,NDV)复制过程中的作用,本研究检测了 NDV NA-1毒株感染10日龄SPF雏鸡和HD11细胞后NLRX1的表达水平,以及病毒在NLRX1过表达或低表达时的NDV的增殖情况、HD11细胞促炎因子的表达情况和氧化应激状态.结果表明:NA-1感染可引起体内外NLRX1表达的增加;NLRX1过表达则会抑制病毒的增殖,提高细胞IL-1β、IL-6和IFN-β的表达.而NLRX1升高不会引起细胞自噬和凋亡细胞水平的变化,但是会使细胞在24 h内iNOS、Keap1、Nrf2、NQO1和HO-1的mRNA水平均升高.综上,NLRX1可能通过促进早期ROS的产生从而抑制NDV的增殖.
NLRX1 inhibits NDV proliferation by promoting ROS secretion
To investigate the role of the nucleotide-binding domain and leucine-rich-repeat-contai-ning family member X1(NLRX1)in the replication of Newcastle disease virus(NDV),the ex-pression level of NLRX1 was examined following infection of 10-day-old specific pathogen-free(SPF)chicks and HD11 cells with the NDV NA-1 strain.Additionally,the proliferation of NDV,the expression of pro-inflammatory cytokines in HD11 cells,and the oxidative stress status were e-valuated in the presence of either overexpressed or underexpressed NLRX1.The results demonstra-ted that NA-1 infection led to an increase in NLRX1 expression both in vivo and in vitro.Further-more,overexpression of NLRX1 inhibited viral proliferation,enhanced the expression of cellularIL-1β,IL-6,and IFN-β,without affecting levels of autophagy or apoptotic cells.However,NLRX1 elevation resulted in elevated mRNA levels of iNOS,Keap1,Nrf2,NQO1,and HO-1 within 24 h.In conclusion,NLRX1 suppresses NDV proliferation by promoting early ROS production.
Newcastle disease virusnucleotide-binding domain and leucine-rich-repeat-containing family member X1intracellular inducible nitric oxide synthasereactive oxygen species
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