山羊地方性鼻内肿瘤病毒安徽株全基因组序列分析
Whole-genome sequence analysis of Anhui strain of enzootic nasal tumor virus in goats
李翎旭 1王珍 1郭文晴 1林子彦 1常广军 1姚大伟1
作者信息
- 1. 南京农业大学动物医学院,江苏南京 210095
- 折叠
摘要
获得安徽地区2株山羊地方性鼻内肿瘤病毒(enzootic nasal tumor virus-2,ENTV-2)全基因组序列,分析EN-TV-2 基因的遗传多样性.采集安徽某羊场6只患地方性鼻内腺癌(enzootic nasal adenocarcinoma,ENA)的山羊鼻腔分泌物和血液样品,采用Trizol法提取总RNA,去除其中DNA干扰完成两步法反转录,PCR检测病料中的EN-TV-2.选择其中2只山羊的阳性样品,采用5对引物扩增ENTV-2全基因组序列,测序拼接后上传NCBI数据库,与数据库中序列进行比较分析并构建遗传进化树.本研究在6只病羊鼻腔分泌物样品中均可检测出ENTV-2,而血液中不能检出.ENTV-2基因全长约7 400 bp,分别命名为ENTV-2AH1(DDBJ登录号:LC762616)和ENTV-2AH2(DDBJ登录号:LC762617),序列分析显示与国内福建株(ENTV-2FJ)、广西株(ENTV-2-DA0)同源性分别为99.2％、99.1％,并且处于同一进化分支.本研究首次在安徽地区检测到山羊地方性鼻内肿瘤病毒,获得了 ENTV-2全基因组序列,有助于进一步研究国内ENTV-2遗传多样性.
Abstract
This study aims to obtain two whole-genome sequences of enzootic nasal tumor virus of goats(ENTV-2)from Anhui Province and analyzed the genetic diversity of ENTV-2 gene.Nasal secretion samples and blood samples of six goats with enzootic nasal adenocarcinoma(ENA)were collected from a goat farm in Anhui Province.The total RNA was extracted by the TRIzol method.The DNA interference was removed by the two-step reverse transcription.The ENTV-2 was detec-ted by PCR.Then,two positive samples were selected and five pairs of primers were used to ampli-fy the whole-genome sequences of ENTV-2.After sequencing and splicing,two sequences were up-loaded to the database for comparative analysis with the sequences in the NCBI database.Finally,the genetic evolution tree was constructed.ENTV-2 was detected in the nasal secretion samples,but not in the blood of the six ENA goats.The ENTV-2 genes were approximately 7 400 bp in length,named ENTV-2AH1(DDBJ accession no.:LC762616)and ENTV-2AH2(DDBJ accession no.:LC762617),respectively.Two sequences showed 99.2％and 99.1％homology with the Fujian strain(ENTV-2FJ)and Guangxi strain(ENTV-2-DA0),respectively.They were in the same evo-lutionary branch.In this study,two whole-genome sequences of ENTV-2 were obtained in Anhui for the first time,which can help to further study the genetic diversity of ENTV-2 in China.
关键词
山羊/地方性鼻内肿瘤病毒/地方性鼻内腺癌/全基因组序列Key words
goat/enzootic nasal tumor virus/enzootic nasal adenocarcinoma/whole-genome sequence引用本文复制引用
出版年
2024
NETL
NSTL
万方数据