鹦鹉蛔虫的鉴定及其线粒体基因组的分析
Identification of parrot roundworms and analysis of its mitochondrial genome
李娜 1任照文 1王娅妮 2张翩 2孙通宝 2袁子国 3王晓虎2
作者信息
- 1. 华南农业大学兽医学院,广东 广州 510640;广东省农业科学院动物卫生研究所广东省畜禽疫病防治研究重点实验室,广东 广州 510640
- 2. 广东省农业科学院动物卫生研究所广东省畜禽疫病防治研究重点实验室,广东 广州 510640
- 3. 华南农业大学兽医学院,广东 广州 510640
- 折叠
摘要
旨在通过形态学分析和分子生物学技术鉴定来自广东地区的折衷鹦鹉和大绯胸鹦鹉肠道内蛔虫,并使用PCR技术扩增鹦鹉蛔虫的线粒体基因组,同时基于其线粒体基因序列进行了系统发育分析.结果显示,在本次鉴定中发现的鹦鹉蛔虫为Ascaridia nymphii,其线粒体基因组包括12个蛋白质编码基因,22个tRNA基因,2个核糖体RNA基因以及2个非编码区.系统发育分析显示,本研究鉴定到的A.nymphii与先前报道的Ascaridia sp.位于同一进化分支,具有较近的亲缘关系,并与鸽蛔虫和鸡蛔虫构成一个独立的禽蛔虫支系.这一研究为鹦鹉蛔虫的分类、分子流行病学和种群遗传进化提供了重要的数据支持.
Abstract
To identify the intestinal roundworms of Eclectus roratus and Psittacula alexandri from the Guangdong region by morphological analysis and molecular biology techniques,and to amplify the mitochondrial genome of parrot roundworms using PCR,as well as to carry out phylogenetic analyses based on the sequences of their mitochondrial genes.The results showed that the parrot roundworms identified in this identification belongs to Ascaridia.nymphii and its mitochondrial genome consists of 12 protein coding genes,22 tRNA genes,2 ribosomal RNA genes and two non-coding regions.Phylogenetic analyses showed that A.nymphii identified in this study is located in the same evolutionary branch as the previously reported Ascaridia sp.,is closely related and con-stitutes a separate clade of avian ascarids with pigeon roundworms and chicken roundworms.This study provides important data for the classification,molecular epidemiology and population genetic evolution of parrot roundworms.
关键词
鹦鹉蛔虫/线粒体基因/系统发育分析Key words
parasitic roundworm/mitochondrial genes/phylogenetic analysis引用本文复制引用
出版年
2024
NETL
NSTL
万方数据