二甲双胍与三氧化二砷对KG1a细胞增殖的抑制作用
Inhibitory Effect of Metformin and Arsenic Trioxide on KG1a Cell Proliferation
黄李文惠 1刘萌 1桂淑敏 1冯明明 1刘慧 1司晓慧 1牛新清1
作者信息
- 1. 新乡医学院医学技术学院;河南省免疫与靶向药物重点实验室;河南省分子诊断与医学检验技术协同创新中心,河南新乡453003
- 折叠
摘要
目的:探讨二甲双胍协同三氧化二砷对急性髓系白血病KG1a细胞增殖的影响及其可能的机制.方法:采用CCK-8法检测二甲双胍、三氧化二砷以及联合应用对KG1a细胞的杀伤作用;Annexin V-FITC/PI双染法流式细胞术检测应用二甲双胍协同三氧化二砷对KG1a细胞凋亡的影响;Western blot检测胞内凋亡、自噬相关蛋白的表达.结果:二甲双胍与三氧化二砷联合及单独应用均可抑制KG1a细胞增殖,诱导KG1a细胞凋亡,联合用药组增殖抑制率及凋亡率高于单独用药组(P＜0.05).联合用药诱导KG1a细胞中Caspase 8和P62蛋白表达上调且高于单药组(P＜0.05).结论:二甲双胍能协同三氧化二砷杀伤KG1a细胞,其作用机制可能与诱导凋亡和增强自噬有关.
Abstract
Objective:To investigate the effect of metformin and arsenic trioxide on KG1a cells proliferation of acute myeloid leukemia and its possible mechanism.Methods:CCK-8 method was used to detect the killing effect of metformin,arsenic trioxide and combined application on KG1a cells.Annexin V-FITC/P1 Dual Stain Flow Cytometry was used to detect the effect of combined application on apoptosis of KG1 a cells.Western blot was used to detect the expression of intracellular apoptosis-,autophagy-related protein.Results:Metformin and arsenic trioxide alone or in combination could inhibit the proliferation of KG1 a cells and induce apoptosis of KG1 a cells,and the proliferation inhibition rate and apoptosis rate in the combined drug group were higher than those in the drug group alone(P＜0.05).The combination of drugs induced upregulation of Caspase 8 protein and P62 protein expression and was higher than that in the drug group alone(P＜0.05).Conclusion:Metformin can synergize with arsenic trioxide to kill KG1a cells,and its mechanism of action may be related to inducing apoptosis and enhancing autophagy.
关键词
三氧化二砷/二甲双胍/急性髓系白血病/Caspase/8/P62Key words
arsenic trioxide/metformin/acute myeloid leukemia/Caspase 8/P62引用本文复制引用
基金项目
国家自然科学基金(81800139)
河南省自然科学基金(182300410300)
高等学校学科创新引智计划(111计划)(D20036)
出版年
2024
NETL
NSTL
维普
万方数据