γδT17/Th17/Tc17细胞在维吾尔族慢性淋巴细胞白血病患者外周血中的比例及临床意义
Proportion and Clinical Significance of γδT17/Th17/Tc17 Cells in Peripheral Blood of Uygur Patients with Chronic Lymphoblastic Leukemia
曾雪娇 1张瑞 1谢仁古丽·阿力木 1玛利娅·木哈什 1赵芳 1古丽·巴旦木 1曲建华1
作者信息
- 1. 新疆医科大学第一附属医院血液病中心,新疆维吾尔族自治区血液病研究所,新疆乌鲁木齐 830054
- 折叠
摘要
目的:探讨维吾尔族慢性淋巴细胞白血病(CLL)患者外周血中γδT17/Th17/Tc17细胞的比例分布及临床意义.方法:选取53例初诊维吾尔族CLL患者及30例健康者,采用ELISA法检测患者和对照者外周血血清中IL-17、IL-23、IL-6、IFN-γ的水平,采用流式细胞术检测维吾尔族CLL患者与对照组外周血中γδT/γδT17/Th17/Tc17细胞的比例,观察上述指标在CLL Binet分期中的变化差异.结果:与对照组相比,维吾尔族CLL患者外周血γδT细胞、γδT17细胞、Th17细胞比例均明显增高(P＜0.05),其中γδT17细胞占总淋巴细胞比例较Th17细胞、Tc17细胞均明显增高(P＜0.05),且随着疾病分期进展,γδT及γδT17细胞比例均增高(P＜0.05).维吾尔族CLL患者外周血IL-17、IL-23、IL-6均较对照组明显增高(P＜0.05),而细胞因子IFN-γ的水平较对照组明显减低(P＜0.05),且随着疾病分期进展,外周血IL-17水平逐渐减低(P＜0.05).结论:γδT及γδT17在维吾尔族CLL中异常高表达,并与疾病分期有关,参与了CLL发生发展.
Abstract
Objective:To explore the distribution of γδT17/Th17/Tc17 cells in the peripheral blood of Uygur patients with chronic lymphoblastic leukemia(CLL)and clinical significance.Methods:ELISA method was used to detect the levels of IL-17,IL-23,IL-6,and IFN-γ in the peripheral blood serum of 53 newly diagnosed Uygur patients with CLL and 30 healthy controls.Flow cytometry was used to determine the proportion of γδT/γδT17/Th17/Tc17 cells in the peripheral blood of Uygur CLL patients and controls,and the changes of the abover indexes in CLL Binet staging were observed.Results:Compared with the control group,the proportion of γδT cells,γδT17 cells,and Th17 cells in the peripheral blood of Uygur CLL patients increased significantly(P＜0.05). δT17 cell proportion in total lymphocytes was significantly higher than Th17 and Tc17 cell proportions(P＜0.05),and proportions of γδT,yδT17 cells increased gradually as the disease progressed.The levels of cytokines IL-17,IL-23,and IL-6 in peripheral blood of Uygur patients with CLL were significantly higher than those in the control group(P＜0.05),while the level of cytokine IFN-γ was significantly lower(P＜0.05).The level of IL-17 in peripheral blood decreased gradually as the disease progressed(P＜0.05).Conclusion:γδT and γδT17 are abnormally highly expressed in Uygur CLL,which are related to the stage of disease and participate in the occurrence and development of CLL.
关键词
慢性淋巴细胞白血病/γδT细胞/IL-17Key words
chronic lymphoblastic leukemia/γδT cell/IL-17引用本文复制引用
基金项目
国家自然科学基金(82160034)
国家自然科学基金(81860033)
自治区科技计划(2022D14008)
出版年
2024
NETL
NSTL
维普
万方数据