Proportion and Clinical Significance of γδT17/Th17/Tc17 Cells in Peripheral Blood of Uygur Patients with Chronic Lymphoblastic Leukemia
Objective:To explore the distribution of γδT17/Th17/Tc17 cells in the peripheral blood of Uygur patients with chronic lymphoblastic leukemia(CLL)and clinical significance.Methods:ELISA method was used to detect the levels of IL-17,IL-23,IL-6,and IFN-γ in the peripheral blood serum of 53 newly diagnosed Uygur patients with CLL and 30 healthy controls.Flow cytometry was used to determine the proportion of γδT/γδT17/Th17/Tc17 cells in the peripheral blood of Uygur CLL patients and controls,and the changes of the abover indexes in CLL Binet staging were observed.Results:Compared with the control group,the proportion of γδT cells,γδT17 cells,and Th17 cells in the peripheral blood of Uygur CLL patients increased significantly(P＜0.05). δT17 cell proportion in total lymphocytes was significantly higher than Th17 and Tc17 cell proportions(P＜0.05),and proportions of γδT,yδT17 cells increased gradually as the disease progressed.The levels of cytokines IL-17,IL-23,and IL-6 in peripheral blood of Uygur patients with CLL were significantly higher than those in the control group(P＜0.05),while the level of cytokine IFN-γ was significantly lower(P＜0.05).The level of IL-17 in peripheral blood decreased gradually as the disease progressed(P＜0.05).Conclusion:γδT and γδT17 are abnormally highly expressed in Uygur CLL,which are related to the stage of disease and participate in the occurrence and development of CLL.
NETL
NSTL
万方数据
维普
