Curcumin Combined with Thalidomide Inhibits Proliferation of KG-1 Cells and Its Related Mechanisms
Objective:To investigate the effects of curcumin combined with thalidomide on the proliferation and apoptosis of acute myeloid leukemia KG-1 cells,and its correlation with B-cell lymphoma-xL(Bcl-xL),signal transducer and activator of transcription 3(STAT3).Methods:MTT assay was used to detect the proliferation of KG-1 cells and screen the optimal combined concentration of curcumin and thalidomide.The effects of curcumin,thalidomide and their combination on the proliferation and apoptosis of KG-1 cells were analyzed by MTT method and flow cytometry,respectively.The mRNA expression levels of STAT3 and Bcl-xL in single-drug group,two-drug combination group and control group(untreated cells)were detected by real-time quantitative PCR.Results:Both curcumin and thalidomide inhibited the proliferation of KG-1 cells in a concentration-dependent manner in the range of 20-100 μmol/L(r=0.657,r=0.681).The IC50 value of curcumin and thalidomide at 48 h was(42.07±0.50)μmol/L and(57.01±2.39)μmol/L,respectively.The cell proliferation inhibition rate of curcumin(40 μmol/L)+thalidomide(60 μmol/L)was(86.67±1.53)％,which was significantly higher than(51.67±1.15)％of curcumin(40 μmol/L)and(55.33±1.53)％of thalidomide(60 μmol/L)(both P＜0.05).Treated with curcumin and thalidomide alone or in combination,the apoptosis rate of KT-1 cells was(18.67±2.08)％,(21.33±2.52)％,and(46.67±1.53)％,respectively,which was significantly higher than(0.72±0.03)％of control group(all P＜0.05).The cell apoptosis rate of two-drug combination group was significantly higher than that of single-drug group(both P＜0.05).Compared with the control group,the mRNA expressions of STAT3 and Bcl-xL in single-drug group,two-drug combination group were significantly decreased(both P＜0.05).Compared with single-drug group,the mRNA expressions of STAT3 and Bcl-xL in two-drug combination group were also significantly decreased(both P＜0.05).Conclusion:Curcumin combined with thalidomide can synergistically down-regulate the expression of STAT3 and Bcl-xL,inhibit the proliferation of KG-1 cells,and induce apoptosis.
acute myeloid leukemiacurcuminthalidomideB-cell lymphoma-xLsignal transducer and activator of transcription 3
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