LINC00475在多发性骨髓瘤中的表达与临床意义
Expression and Clinical Significance of LINC00475 in Multiple Myeloma
卢玲 1郭丹 1洪乐昇 1蒋钰雯 1范洪明 1黄春群 1卢金凤 1陈洁 1徐宏慧 1黄红铭1
作者信息
- 1. 南通大学附属医院血液内科,江苏南通 226001
- 折叠
摘要
目的:探讨LINC00475在多发性骨髓瘤(MM)患者血清中的相对表达水平及临床意义.方法:用实时荧光定量PCR技术检测108例 MM患者血清及RPMI 8226、NCI-H929、U266、OPM2、CAG这5个MM细胞系中LINC00475的表达情况,受试者工作特征曲线评估其对MM的诊断价值,并结合患者的临床资料分析LINC00475与患者临床特征的关系.结果:与健康对照组相比,MM患者血清及MM细胞系中LINC00475的表达明显上调(均P＜0.05).受试者工作特征曲线分析显示,LINC00475的最佳截断值为262.4,曲线下面积为0.924(95％CI:0.884-0.964),灵敏度83.3％,特异度91.7％,表明LINC00475在MM患者中具有良好的评估价值.与LINC00475低表达组的MM患者相比,高表达组的患者具有较高水平的β2-微球蛋白和胱抑素C(均P＜0.05),但白蛋白水平较低(P＜0.05).与ISS分期Ⅰ期的MM患者相比,Ⅱ期和Ⅲ期患者LINC00475的表达水平均明显增高(均P＜0.05).结论:检测LINC00475的相对表达水平有助于区分MM患者和正常人群,其相对表达水平与MM患者的β2-微球蛋白、白蛋白、ISS分期等预后指标相关.
Abstract
Objective:To investigate the relative expression level and clinical significance of LINC00475 in serum of patients with multiple myeloma(MM).Methods:The expression of LINC00475 in serum of 108 MM patients and five MM cell lines including RPMI 8226,NCI-H929,U266,OPM2 and CAG were detected by real-time fluorescence quantitative PCR.The diagnostic value of LINC00475 in MM was evaluated by receiver operating characteristic(ROC)curve analysis.The correlation of LINC00475 with patients'characteristics was analyzed.Results:Compared with control groups,the expression of LINC00475 was up-regulated in serum of MM patients and MM cell lines(all P＜0.05).ROC curve analysis showed that the optimal cut-off value of LINC00475 was 262.4,the area under curve(AUC)was 0.924(95％CI:0.884-0.964),and sensitivity and specificity was 83.3％and 91.7％,respectively,which indicated that LINC00475 had good evaluation value in MM patients.Compared with low-LINC00475 expression group,patients in high-LINC00475 expression group had higher levels of β2-microglobulin(β2-MG)and Cystatin C(Cys-C)but lower albumin(ALB)(all P＜0.05).Compared with MM patients with International Staging System(ISS)stage I,the expression level of LINC00475 was significantly higher in patients with stage Ⅱ and Ⅲ(both P＜0.05).Conclusion:LINC00475 is helpful to distinguish MM patients from healthy adults,which is correlated with the prognostic indicators such as β2-MG,ALB,and ISS stage.
关键词
多发性骨髓瘤/LINC00475/长链非编码RNAKey words
multiple myeloma/LINC00475/long non-coding RNA引用本文复制引用
基金项目
国家自然科学基金青年科学基金(82200219)
南通市科技计划(JC12022095)
出版年
2024
NETL
NSTL
维普
万方数据