首页|XPO1抑制剂塞利尼索对急性髓系白血病Kasumi-1细胞增殖和凋亡的影响

XPO1抑制剂塞利尼索对急性髓系白血病Kasumi-1细胞增殖和凋亡的影响

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目的:探讨核输出蛋白1(XPO1)抑制剂塞利尼索对急性髓系白血病(AML)Kasumi-1细胞增殖与凋亡的影响.方法:用MTS法检测不同浓度塞利尼索(0、2.5、5、10 μmol/L)作用于Kasumi-1细胞不同时间点(24、48、72 h)的增殖抑制率;流式细胞术检测不同浓度塞利尼索作用于Kasumi-1细胞48 h后的细胞凋亡率和细胞周期变化情况.结果:塞利尼索在不同时间点均可抑制Kasumi-1细胞生长,并呈浓度依赖(r24 h=0.7592,r48 h=0.9456,r72 h=0.9425);不同浓度塞利尼索均可抑制Kasumi-1细胞生长,并呈时间依赖(2.5 μmol/L组r=0.9057,5μmol/L组r=0.9897,10 μmol/L组r=0.9994).塞利尼索可诱导Kasumi-1细胞凋亡,且呈浓度依赖(r=0.9732),随着药物浓度的增大,诱导Kasumi-1细胞凋亡的作用越明显.塞利尼索作用于Kasumi-1细胞48 h后,G0/G1期细胞比例明显上升,S期细胞比例明显下降,并且随着塞利尼索药物浓度的增大,Kasumi-1细胞周期阻滞于G0/G1期的细胞比例增加,细胞合成减少.结论:塞利尼索可抑制Kasumi-1细胞增殖,诱导细胞凋亡,阻滞细胞周期于G0/G1期,导致肿瘤细胞的死亡.
Effect of Selinexor on Proliferation and Apoptosis of Acute Myeloid Leukemia Kasumi-1 Cells
Objective:To investigate the effects of selinexor,a inhibitor of nuclear export protein 1(XPO1)on the proliferation inhibition and apoptosis of Kasumi-1 cells in acute myeloid leukemia(AML).Methods:MTS method was used to detect the inhibitory effect of different concentrations of selinexor on the proliferation of Kasumi-1 cells at different time points.The apoptosis rate and cell cycle changes after treatment with different concentration of selinexor were detected by flow cytometry.Results:Selinexor inhibited the growth of Kasumi-1 cells at different time points in a concentration-dependent manner(r24 h=0.7592,r48 h=0.9456,and r72 h=0.9425).Selinexor inhibited Kasumi-1 cells growth in a time-dependent manner(r=0.9057 in 2.5 µmol/L group,r=0.9897 in 5 μmol/L group and r=0.9994 in 10 μmol/L group).Selinexor could induce apoptosis of Kasumi-1 cells in a dose-dependent manner(r=0.9732),and the apoptosis of Kasumi-1 cells was more obvious with the increase of drug concentration.The proportion of G0/G1 phase was significantly increased and the proportion of S phase was significantly decreased after the treatment of Kasumi-1 cells by selinexor.With the increase of drug concentration,the proportion of Kasumi-1 cells cycle arrest in G0/G1 phase was increased and the cell synthesis was decreased.Conclusion:Selinexor can promote the death of tumor cells by inhibiting Kasumi-1 cells proliferation,inducing apoptosis and blocking cell cycle.

XPO1selinexorkasumi-1 cellsproliferationapoptosis

林璐慧、高孙巧、梅序桥、林达义、陈艺凤、林素丹、庄丽鸿、林聪猛

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福建医科大学附属漳州市医院血液科,福建漳州 363000

福建医科大学附属漳州市医院检验科,福建漳州 363000

核输出蛋白1 塞利尼索 Kasumi-1细胞 增殖 凋亡

福建省自然科学基金福建省自然科学基金福建省自然科学基金漳州市自然科学基金漳州市医院博士工作室项目基金漳州市医院博士工作室项目基金漳州市医院博士工作室项目基金福建省卫健委科技计划项目

2020J0113032020J0112992020J011302ZZ2020J05PDA202103PDA202104PDB2021102023CXA053

2024

10.19746/j.cnki.issn1009-2137.2024.04.017

中国实验血液学杂志
中国病理生理学会

中国实验血液学杂志

CSTPCD北大核心
影响因子：0.988
ISSN：1009-2137
年,卷(期)：2024.32(4)
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