PD-1/PD-L1在MDS原始细胞、T细胞亚群和Treg细胞中的表达及意义
Expression and Significance of PD-1/PD-L1 in MDS Blast Cells,T Lymphocyte Cell Subsets and Treg Cells
王跃飞 1陈葆国 2郑瑞 2俞依妮2
作者信息
- 1. 浙江大学附属二院临海分院(临海市第一人民医院)儿内科
- 2. 温州医科大学附属台州医院中心实验室,浙江临海 317000
- 折叠
摘要
目的:探讨PD-1/PD-L1在MDS原始细胞、T细胞亚群和Treg细胞中的表达及其意义.方法:以88例MDS患者和19例AML患者为研究对象,以缺铁性贫血和健康骨髓捐献者为对照.采用流式细胞术分别检测其MDS/AML原始细胞、T细胞亚群和Treg细胞中PD-1/PD-L1的表达,同时检测外周血清Th1/Th2/Th17相关细胞因子的表达水平.结果:低危MDS组原始细胞、T细胞亚群、Treg细胞中PD-1/PD-L1的表达水平均明显低于对照组、中高危MDS组和AML组(均P＜0.01);细胞因子以Th1/Th17类型占优势.中高危MDS组和AML组原始细胞、T细胞亚群、Treg细胞中的PD-1/PD-L1表达均明显高于对照组和低危MDS组(均P＜0.01);细胞因子以Th2类型和Treg类型(IL-10、TGF-β)占优势.治疗后,MDS缓解组原始细胞、T细胞亚群、Treg细胞中PD-1/PD-L1的表达较对照组均无明显差异(P＞0.05).MDS未缓解组原始细胞、T细胞亚群、Treg细胞中PD-1/PD-L1的表达均显著高于MDS缓解组和对照组(P＜0.01).结论:PD-1/PD-L1在MDS患者中存在高表达,Treg(IL-10、TGF-β)和Th2相关细胞因子占优势以及效应T细胞被抑制的微环境,有利于肿瘤细胞增殖和免疫逃逸,可能促进了 MDS疾病的进展.
Abstract
Objective:To investigate the expression and significance of PD-1/PD-L1 in MDS blast cells,T lymphocyte cell subsets and Treg cells.Methods:Eighty-eight MDS patients and 19 AML patients were collectd as the study subjects,and Iron deficiency anemia and healthy bone marrow donors were used as control group.The expression of PD-1/PD-L1 in MDS/AML blast cells,T lymphocyte cell subsets and Treg cells was detected by flow cytometry,and the expression level of Th1/Th2/Th17-related cytokines in peripheral serum was detected.Results:The expression of PD-1/PD-L1 in blast cells,T lymphocyte cell subsets and Treg cells in low risk MDS group were lower than that in control group,medium and high risk MDS group and AML group(all P＜0.01),and Th1/Th17 type cytokines were dominant.The expression of PD-1/PD-L1 in blast cells,T lymphocyte cell subsets and Treg cells of intermediate and high risk MDS group and AML group were higher than that of control group and low risk MDS group(all P＜0.01),and Th2 type and Treg type(IL-10、TGF-β)cytokines were dominant.After treatment,the differences of PD-1/PD-L1 expression were not statisticatly significant in blast cells,T lymphocyte cell subsets and Treg cells between the MDS remission group and the control group(all P＞0.05).The expression of PD-1/PD-L1 in blast cells,T lymphocyte cell subsets and Treg cells in MDS non-remission group were significantly higher than that in remission group and control group(all P＜0.01).Conclusion:The high expression of PD-1/PD-L1,dominance of Treg(IL-10、TGF-β)and Th2-related cytokines and inhibition of effector T lymphocyte cells in patients with MDS is conducive to tumor cell proliferation and immune escape,which may promote the progression of MDS disease.
关键词
骨髓增生异常综合征/PD-1/PD-L1/T细胞亚群/Treg细胞Key words
myelodysplastic syndromes/PD-1/PD-L1/T lymphocyte cell subsets/Treg cells引用本文复制引用
出版年
2024
NETL
NSTL
万方数据