首页|白藜芦醇对人骨髓间充质干细胞自发衰老的抑制作用

白藜芦醇对人骨髓间充质干细胞自发衰老的抑制作用

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  • NETL
  • NSTL
  • 万方数据
目的:观察白藜芦醇(RSV)对人骨髓间充质干细胞(MSC)的自发衰老是否具有抑制作用.方法:将MSC传至p13和p15代,建立MSC的自发衰老模型组;用5 nmol/L RSV分别处理p13和p15代MSC 48 h,建立RSV处理组.通过SA-β-Gal染色法检测细胞衰老,MTT法检测细胞增殖,RT-PCR检测细胞衰老相关端粒酶活性,Western blot检测与衰老相关的磷酸化mTOR的水平.结果:SA-β-Gal染色显示,RSV处理组MSC的衰老细胞数量明显少于模型组(p13代MSC,RSV组ts模型组,P＜0.05;p15代MSC,RSV组vs模型组,P＜0.01).MTT结果显示,RSV处理组MSC的增殖能力高于模型组,其中p13代MSC处理组与模型组在72 h相比有显著差异(P＜0.05).RT-PCR结果显示,RSV处理组中MSC的hTERT mRNA表达量高于模型组,其中p13代MSC的RSV处理组与模型组相比有显著差异(P＜0.05).Western blot结果显示,RSV处理组中MSC的磷酸化(Ser2448)mTOR水平低于模型组,其中p13代MSC的处理组与模型组相比有显著差异(P＜0.05).结论:RSV可通过调节mTOR活性抑制人MSC的自发衰老.
Inhibition Effect of Resveratrol on Spontaneous Senescence of Human Bone Marrow-Derived Mesenchymal Stem Cells
Objective:To explore whether Resveratrol(RSV)can inhibit the spontaneous senescence of human bone marrow-derived mesenchymal stem cells(MSC).Methods:MSC were serially cultured to passage 13 and passage 15 to establish model groups exhibiting spontaneous senescence,respectively.MSC at passage 13 and passage 15 were treated with 5 nmol/L RSV for 48 h to establish the RSV-treated groups.SA-β-Gal staining was used to detect cell senescence.MTT assay was used to detect cell proliferation.RT-PCR method was used to detect senescence-associated telomerase activity.Western blot was used to detect the senescence-associated protein level of the phosphorylated-mTOR.Results:SA-β-Gal staining showed that the senescent cells of MSC in RSV-treated group was significantly less than those in the model group(RSV group compared with model group at passage 13,P＜0.05;RSV group compared with model group at passage 15,P＜0.01).The cell proliferation ability of MSC in RSV-treated group was significantly higher than those in model group,at 72 h in passage 13,there was significant difference between RSV-treated group and model group(P＜0.05).RT-PCR results showed that the hTERT mRNA expression of MSC in RSV-treated group was higher than that in model group,which was significantly different between RSV-treated group and model group at passage 13(P＜0.05).Western blot results showed that the phosphorylated(Ser2448)-mTOR level of MSC in RSV-treated group was lower than that in model group,which was significantly different between RSV-treated group and model group at passage 13(P＜0.05).Conclusion:RSV can inhibit the spontaneous senescence of human MSC by mediating mTOR activity.

human bone marrow-derived mesenchymal stem cellresveratrolspontaneous senescencehTERTmTOR

杨玥、何建新

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西北民族大学医学部

甘肃中医药大学基础医学院

甘肃中医药大学甘肃省中药药理与毒理学重点实验室,甘肃兰州 730000

人骨髓间充质干细胞 白藜芦醇 自发衰老 hTERT mTOR

国家自然基金青年基金甘肃省自然科学基金甘肃中医药大学敦煌医学与转化重点实验室开放基金西北民族大学引进人才科研项目甘肃省高校青年博士基金

815033771310RJZA083DHYX18-10xbmuyjrc2022352022QB-027

2024

10.19746/j.cnki.issn1009-2137.2024.04.039

中国实验血液学杂志
中国病理生理学会

中国实验血液学杂志

CSTPCD北大核心
影响因子：0.988
ISSN：1009-2137
年,卷(期)：2024.32(4)