摘要
目的:探讨急性髓系白血病(AML)患者血清组蛋白去乙酰化酶2(HDAC2)的表达及其临床意义.方法:纳入2017年4月-2019年4月在本院接受治疗的150例AML患者为AML组,并进一步按生存情况分为生存组108例、死亡组42例.另纳入同期健康体检者100例为对照组.采用实时定量PCR检测患者血清HDAC2表达水平;采用Cox回归分析患者不良预后的影响因素;采用受试者工作特征(ROC)曲线评价血清HDAC2对患者不良预后的预测效能.结果:与对照组相比,AML组患者血清中HDAC2、白介素(IL)-6、肿瘤坏死因子-α(TNF-α)、IL-1β、C反应蛋白(CRP)的表达显著上升(均P＜0.05).与生存组相比,死亡组患者血清中HDAC2的表达亦显著上升(P＜0.05).AML 患者血清 HDAC2 与 IL-6、TNF-α、IL-1β 和 CRP 水平呈正相关(r=0.567、0.559、0.623、0.537).Cox 回归分析结果显示,IL-6、TNF-α、IL-1β、HDAC2均是影响AML患者预后的独立危险因素(均P＜0.05).ROC分析结果显示,AML患者血清HDAC2水平预测AML不良预后的AUC值为0.862.结论:AML患者血清HDAC2表达水平升高,与IL-6、TNF-α、IL-1β和CRP水平呈正相关,是AML患者不良预后的独立危险因素,对AML患者不良预后的预测价值较高.
Abstract
Objective:To investigate the expression of serum histone deacetylase 2(HDAC2)in patients with acute myeloid leukemia(AML)and its clinical significance.Methods:The 150 AML patients who received treatment in our hospital from April 2017 to April 2019 were included as AML group,and further divided into survival group(108 cases)and death group(42 cases)according to their survival status.In addition,100 health individuals undergoing health examination in the same period were included as control group.The expression of HDAC2 in serum was detected by real-time quantitative PCR.Cox regression was used to analyze the influencing factors of adverse prognosis.The predictive effect of serum HDAC2 for the adverse prognosis of AML patients was evaluated by the receiver operating characteristic(ROC)curve.Results:Compared with the control group,the expression of serum HDAC2,interleukin(IL)-6,tumor necrosis factor(TNF)-α,IL-1β and C-reactive protein(CRP)in AML group was obviously increased(all P＜0.05).Compared with the survival group,the expression of serum HDAC2 in the death group was also increased(P＜0.05).Serum HDAC2 was positively correlated with IL-6,TNF-α,IL-1β and CRP in AML patients(r=0.567,0.559,0.623,0.537).According to Cox regression analysis,the IL-6,TNF-α,IL-1 β and HDAC2 were independent risk factors affecting the prognosis of AML patients(all P＜0.05).ROC analysis showed that the AUC of serum HDAC2 level in predicting the adverse prognosis of AML was 0.862.Conclusion:The expression level of serum HDAC2 in AML patients is increased,which has positive correlations with IL-6,TNF-α,IL-1β and CRP.HDAC2 is an independent risk factor for the poor prognosis of AML patients,and has a high predictive value.
维普
万方数据