首页|基于转录组学的原发性血小板增多症血小板活化相关基因分析

基于转录组学的原发性血小板增多症血小板活化相关基因分析

扫码查看
目的:基于转录组测序技术分析原发性血小板增多症(ET)血小板活化相关基因,寻找与ET血栓形成相关的潜在靶点.方法:收集ET患者和健康人的血液标本进行转录组测序,选取差异表达的lncRNA、miRNA和mRNA,构建lncRNA-miRNA-mRNA调控网络.使用基因本体论(GO)和京都基因与基因组百科全书(KEGG)对调控网络中的差异mRNA进行富集分析.应用实时荧光定量PCR(Real-time PCR)方法验证关键信号通路上的差异mRNA.结果:共鉴定出差异表达的32个lncRNA(3个上调、29个下调)、16个miRNA(8个上调、8个下调)和35个mRNA(27个上调、8个下调).其中5个lncRNA、12个miRNA和19个mRNA构成调控网络.KEGG富集分析显示,差异mRNA与血小板活化信号通路相关,与血小板活化相关的差异mRNA有6个,分别为F2R、ITGA2B、ITGB1、ITGB3、PTGS1和GP1 BB,均表达上调.RT-PCR验证结果显示,与健康人相比,ET患者5个mRNA包括F2 R、ITGA2 B、ITGB1、ITGB3和GP1 BB表达水平上调,与转录组测序结果一致,而PTGS1表达无显著差异.结论:ET患者差异mRNA与血小板活化通路相关,F2R、ITGA2B、ITGB1、ITGB3和GP1BB mRNA可作为ET血小板活化相关的新靶点.
Analysis of Genes Related to Platelet Activation in Essential Thrombocythemia Based on Transcriptomics
Objective:To analyze the genes related to platelet activation in essential thrombocythemia (ET)based on transcriptome sequencing technology (RNA-seq ),and to explore the potential targets related to ET thrombosis. Methods:Blood samples from ET patients and healthy individuals were collected for RNA-seq,and differentially expressed lncRNAs,miRNAs,and mRNAs were selected to construct a lncRNA-miRNA-mRNA regulatory network. Differential mRNAs in the regulatory network were enriched and analyzed using Gene Ontology (GO ) and Kyoto Encyclopedia of Genes and Genomes (KEGG).The real-time PCR method was applied to validate differential mRNAs on crucial signaling pathways.Results:A total of 32 lncRNAs (3 up-regulated,29 down-regulated),16 miRNAs (8 up-regulated,8 down-regulated),and 35 mRNAs (27 up-regulated,8 down-regulated)were identified as differentially expressed.Among them,5 lncRNAs,12 miRNAs,and 19 mRNAs constituted the regulatory network.KEGG enrichment analysis showed that the differential mRNAs were related to the platelet activation signaling pathway,and there were 6 differential mRNAs related to platelet activation,namely F2R,ITGA2B,ITGB1,ITGB3,PTGS1,and GP1 BB,which were all up-regulated in their expression.RT-PCR results showed that the expression of five mRNAs including F2R,ITGA2B,ITGB1,ITGB3,and GP1BB were upregulated in ET patients compared with healthy subjects,and consistent with RNA-seq results,while PTGS1 expression was not significantly different.Conclusion:Differential mRNAs in ET patients are related to the platelet activation pathway,and F2R,ITGA2B,ITGB1,ITGB3,and GP1BB mRNAs may serve as novel targets associated with platelet activation in ET.

essential thrombocythemiatranscriptomicslncRNA-miRNA-mRNAplatelet activation

孙妍、杨二鹏、李雨蒙、牛继聪、赵霈、刘为易、陈卓、王明镜、范腾、胡晓梅

展开 >

北京中医药大学研究生院,北京 100029

中国中医科学院西苑医院血液科,北京 100091

中山大学肿瘤防治中心综合中医科,广东广州510060

原发性血小板增多症 转录组学 lncRNA-miRNA-mRNA 血小板活化

2024

中国实验血液学杂志
中国病理生理学会

中国实验血液学杂志

CSTPCD北大核心
影响因子:0.988
ISSN:1009-2137
年,卷(期):2024.32(6)