首页|RhD变异体Type33型受血者同种免疫产生抗-D合并抗-E混合抗体的研究

RhD变异体Type33型受血者同种免疫产生抗-D合并抗-E混合抗体的研究

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目的:探讨1例RhD阳性患者免疫产生抗-D合并抗-E混合抗体的原因.方法:用常规血清学方法鉴定ABO/Rh血型和不规则抗体特异性,采用序列特异性引物聚合酶链式反应(PCR-SSP)检测患者的RHD基因外显子1-10区域并进行杂合性分析,利用一代测序方法分析全外显子序列.结果:患者Rh血型为弱D Type33型,其RHD等位基因为RHD*01W.33,患者表现为RHD+/RHD-杂合型,Rh分型为Ccee,产生了抗-D合并抗-E混合抗体.结论:该患者RHD基因外显子4发生了c.520G>A的突变,这一变异导致了红细胞上RhD抗原的表达减弱.
Study on the Production of Anti-D and Anti-E Mixed Antibodies by Alloimmunization in RhD Variant Type33 Recipients
Objective:To investigate the cause of the production of anti-D and anti-E mixed antibody in an RhD positive patient.Methods:The ABO/Rh blood group typing and irregular antibody specificity were identified by conventional serological methods,the RHD gene exon 1-10 and heterozygous analysis were performed by sequence-specific primer polymerase chain reaction (PCR-SSP),and the whole exon sequence was analyzed by first-generation sequencing.Results:The patient's Rh blood group was weak D Type33,with the allele was RHD*01W.33,the patients was found to be RhD+/RHD-heterozygous,with an Rh typing of Ccee,and the patient had developed anti-D combined with anti-E mixed antibodies.Conclusion:The patient has A c.520G>A mutation in exon 4 of the RHD gene,to lead decreased expression of RhD antigen in red blood cells and the anti-D and anti-E mixed antibodies were produced by transfusion immunostimulation.

weak D type33RhD variantRhD blood groupRHD genemixed antibodyblood receivers

刘建成、邵峰

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宁夏血液中心血型参比实验室,宁夏银川750000

弱D Type33型 RhD变异体 Rh血型 RHD基因 混合抗体 受血者

2024

中国实验血液学杂志
中国病理生理学会

中国实验血液学杂志

CSTPCD北大核心
影响因子:0.988
ISSN:1009-2137
年,卷(期):2024.32(6)